减毒沙门氏菌递呈的TGEVS／N融合基因疫苗的口服免疫原性  

Immunogenicity of attenuated Salmonella typhimurium harbouring S/N fusion gene of porcine transmissible gastroenteritis virus

作　　者：黄小波[1] 张鑫淼[1] 曹三杰[1] 文心田[1] 李春松[1] 梁恩涛 崔婷婷[1] 

机构地区：[1]四川农业大学动物医学院,动物传染病与基因芯片实验室/动物疫病与人类健康四川省重点实验室,雅安625014

出　　处：《中国人兽共患病学报》2013年第6期594-600,共7页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.30901084&No.31072144);教育部高等学校校博士点基金项目(No.20095103120006)联合资助~~

摘　　要：目的研究携带猪传染性胃肠炎病毒(TGEV)S/N融合基因的重组减毒沙门氏菌的口服免疫原性。方法将真核质粒pVAX-S/N电转化鼠伤寒减毒沙门氏菌SL7207,筛选获得疫苗菌株SL7207(pVAX-S/N)。将菌株SL7207(pVAX-S/N)及对照组SL7207(pVAX-S)以1×109 CFU/只灌胃免疫小鼠,测定免疫小鼠诱导的抗TGEV(和抗沙门氏菌均SL7207)的血清IgG及肠道粘膜IgA抗体动态水平,同时测定42d免疫小鼠的干扰素水平。结果菌株SL7207(pVAX-S/N)构建成功,首免后2～6周可诱导产生抗TGEV(和抗沙门氏菌SL7207)的血清IgG,第4～6周可诱导产生抗TGEV(和抗沙门氏菌均SL7207)的肠道粘膜IgA,SL7207(pVAX-S/N)诱导的IgG和IgA抗体最高滴度均略高于SL7207(pVAX-S),但差异不明显(P>0.05)。SL7207(pVAX-S/N)免疫42d小鼠诱导的干扰素浓度为659.45pg/mL,低于SL7207(pVAXD-S)组。结论 S/N融合基因疫苗SL7207(pVAX-S/N)具有良好的口服免疫原性,但与SL7207(pVAX-S)无明显差异。To study the oral immunogenicity of attenuated Salmonella typhimurium harbouring transmissible gastroen teritis virus S/N fusion gene, eukaryotic plasmid pVAX S/N was transformed into attenuate S. typhimurium SL7207 by elec troporation and recombinant strains SL7207 （pVAXS/N） were screened. BLAB/C mice were inoculated intragastrically with 1X 10^9CFU SL7207 （pVAXS/N） and control strains SL7207 （pVAXS）, and the specific serum IgG and intestinal mucosal IgA against TGEV （and against the attenuate S. typhimurium SL7207） in postimmunized mice were detected. The ＂/INF concentration in spleen lymphocyte at 42 day was also detected. The results showed attenuated Salmonella typhimurium SL7207 （pVAXS/N）was constructed correctly. The strain SL7207 （pVAXS/N）could elicit serum IgG and intestines mucosal IgA against TGEV （Salmonella typhimurium SL7207） during week 26 and week 4 6 postimmunization. The highest antibody titer of serum lgG and intestines mucosal IgA induced by SL7207 （pVAXS/N） was slightly higher than that induced by SL7207 （pVAX S）, but there was no significant difference （P）0.05）. The yINF concentration was 659.45 pg/mL in mice spleen lymphocyte at 42 days postimmunization, which was lower than that of SL7207 （pVAXS/N）. The results showed the strain SL7207 （pVAXS/N） harbouring S/N fusion gene had good oral immunogenicity but there was no significantly difference with the SL7207 （pVAX S）.

关 键 词：减毒沙门氏菌 猪传染性胃肠炎病毒 融合双基因 构建 免疫原性 

分 类 号：R378.22[医药卫生—病原生物学]