病毒感染性腹泻患者肛拭样品中诺如病毒分析研究  

Detection of noroviruses from viral infectious diarrhea patients in Jiading , Shanghai

作　　者：郑磊[1] 余玮[1] 顾丽萍[1] 

机构地区：[1]上海市嘉定区疾病预防控制中心,上海201800

出　　处：《中国人兽共患病学报》2013年第6期636-638,共3页Chinese Journal of Zoonoses

摘　　要：目的了解本地区病毒感染性腹泻患者诺如病毒感染情况,进一步探索诺如病毒流行规律和流行因素,为制订防控对策提供依据。方法从2008年3月至2010年2月由嘉定区中心医院采集病毒感染性腹泻患者肛拭样品共572份,采用实时荧光逆转录聚合酶链反应法(Real-Time RT-PCR)检测病毒核酸。结果 572份病毒感染性腹泻患者肛拭样品中RT-PCR检测核酸阳性53例,阳性率为9.27%。结论嘉定区病毒性感染腹泻中有近10%的患者由诺如病毒感染引起,冬季可能为流行高峰,65岁以上为重点人群。The purpose of this study was to investigate prevalence of norovirus infection in patients with viral infectious diarrhea, analyze factors associated with norovirus epidemic, and facilitate control and prevention of norovirus infection in Jiading, Shanghai. A total of 572 anal swabs from patients with viral infectious diarrhea were collected at the Center Hospital in Jiading, March 2008 to February 2010, and detected for norovirus RNA by real-time reverse transcription polymerase chain re action （RT-PCR）. Fifty-three （9.27G） specimens were positive for norovirus RNA. It was indicated by intensive analysis that norovirus infection occurred in the winter, and that population group of the elderly （aged 65＋ years） was more susceptible.

关 键 词：诺如病毒 病毒感染性腹泻 逆转录聚合酶链反应法 

分 类 号：R373.2[医药卫生—病原生物学]