“软脉1号”制剂对小鼠动脉钙化的预防作用  

Ruanmai preparation prevents vascular calcification in mice

作　　者：司徒永立[1] 谢爱媚[1] 郑志明[1] 卢旱云[1] 吴铁[1] 

机构地区：[1]广东医学院药学院药理学教研室,广东省湛江市524023

出　　处：《中国组织工程研究》2013年第20期3730-3737,共8页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技计划项目(2010B06050)~~

摘　　要：背景:原儿茶醛对引起动脉钙化的氧化应激、炎症、钙离子失衡等机制有作用,维生素E对引起动脉钙化氧化应激、免疫紊乱、炎症、血脂异常等机制也有作用,"软脉1号"制剂由原儿茶醛与维生素E两药组成。目的:用超生理剂量维生素D3联合高脂乳剂建立小鼠动脉钙化模型,探讨"软脉1号"制剂对小鼠动脉钙化的预防作用。方法:清洁级KM小鼠40只,随机摸球法均分为正常对照组、模型组、普伐他汀钠组和"软脉1号"制剂组,后3组建立小鼠动脉钙化模型,然后分别给药干预。实验6周后眼球取血处死小鼠,制作胸主动脉苏木精-伊红染色病理切片,观察胸主动脉、血脂、红细胞和血红蛋白的变化。结果与结论:模型组可见胸主动脉有大量黑色钙质沉积于中层弹性纤维中,波浪状的弹性纤维已无法辨清,间距扩大甚至断裂,部分平滑肌细胞坏死消失,内外弹性膜结构不完整,血清总胆固醇、高密度脂蛋白升高(P<0.05),血红蛋白、平均红细胞血红蛋白含量降低(P<0.05)。普伐他汀钠组胸主动脉血管病变无明显改善,总胆固醇、低密度脂蛋白、高密度脂蛋白、血红蛋白、平均红细胞血红蛋白、红细胞数较模型组均无显著性意义。"软脉1号"制剂组小鼠胸主动脉血管病变明显减轻,黑色钙质在弹性纤维中沉积较少,内弹性膜与中层弹性纤维均呈粉染、清晰,弹性纤维间距减少,可见呈波浪状的弹性纤维,内外弹性膜的结构比较完整。与模型组比较,"软脉1号"制剂组总胆固醇下降(P<0.05),血红蛋白、平均红细胞血红蛋白含量升高(P<0.05),其他指标均无统计学意义。说明"软脉1号"制剂对超生理剂量维生素D3联合高脂乳剂造成的小鼠血管钙化具有预防作用。BACKGROUND： Protocatechuic aldehyde treats oxidative stress, inflammation, and calcium imbalance, which cause arterial calcification, while vitamin E functions at oxidative stress, inflammation, immune disorders, and abnormal blood lipid, which also cause arterial calcification. Ruanmai preparation is composed of protocatechuic aldehyde and vitamin E. OBJECTIVE： To establish arterial calcification model of mice with super-physiological dose Vitamin D3 and hyperlipidemia, and to investigate the preventive effect of Ruanmai preparation on arterial calcification in mice. METHODS： Forty clean mice of Kunming species were randomly divided into four groups： normal control group, model group, Pravastatin group and Ruanmai preparation group. Each group contained 10 mice. Except the normal control group, arterial calcification model was established in the other three groups, which was then given drug administrations. Six weeks later, mice were kil ed to col ect blood sample from the eyebal s. Then hematoxylin-eosin staining sections of thoracic aortas were made, to observe the changes of thoracic aortas, blood lipids, red blood cel s and hemoglobin. RESULTS AND CONCLUSION： In the model group, a large number of calcium deposits were seen in the middle elastic fibers of thoracic aorta, wavy elastic fibers were difficult to be distinguished, with expanding space and even fracture, part of smooth muscle cel s were necrotic and disappeared. The structure of internal and external elastic membrane was incomplete. Serum total cholesterol and high-density lipoprotein levels increased （P 〈 0.05）, while hemoglobin and mean corpuscular hemoglobin decreased （P 〈 0.05）. The vascular lesions of thoracic aorta in the Pravastatin group showed no significant improvement. There were no significant differences in the total cholesterol, low-density lipoprotein, high-density lipoprotein, hemoglobin, mean corpuscular hemoglobin and red blood cel s compared with model group. In the Ruanmai preparation group, the vascular le

关 键 词：组织构建 组织构建与中医药 “软脉1号”制剂 普伐他汀钠 维生素D3 高脂乳剂 胸主动脉 动脉钙化 弹性纤维 总胆固醇 低密度脂蛋白 高密度脂蛋白 血红蛋白 小鼠 省级基金 

分 类 号：R318[医药卫生—生物医学工程]