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机构地区:[1]北京大学深圳医院呼吸内科,广东深圳518036 [2]北京大学深圳医院急诊科,广东深圳518036
出 处:《中国医药导报》2013年第18期55-56,59,共3页China Medical Herald
基 金:广东省深圳市科技计划项目(编号200803013)
摘 要:目的探讨铜绿假单胞菌肺炎中内毒素血症与白介素1(IL-1)和肿瘤坏死因子(TNF)的关系。方法 21只BALB/c小鼠随机分成3组,建立小鼠铜绿假单胞菌感染模型,以小剂量多黏菌素B拮抗内毒素活性,观察各组小鼠血浆内毒素水平、肺组织中IL-1βmRNA、TNF-αmRNA水平。结果铜绿假单胞菌感染组小鼠24 h后血浆内毒素水平[(71.2±9.9)EU/mL]明显高于铜绿假单胞菌感染+多黏菌素B组[(50.5±10.8)EU/mL](P<0.01);铜绿假单胞菌感染组小鼠24 h后肺组织中IL-1βmRNA水平[(0.89±0.06)]明显高于铜绿假单胞菌感染+多黏菌素B组[(0.64±0.07)](P<0.01);TNFαmRNA水平[(0.61±0.07)]也明显高于铜绿假单胞菌感染+多黏菌素B组[(0.43±0.06)](P<0.01)。小鼠血浆内毒素水平与肺组织中IL-1β、TNFα含量有显著的相关性(r=0.98,P<0.01;r=0.97,P<0.01)。结论小鼠铜绿假单胞菌肺部感染内毒素血症与IL-1β、TNF-α显著相关。Objective To study the relationship between endotoxemia and interleukin-1β or tumor necrosis factorα in mice with Pseudomonas aeruginosa pneumonia.Methods BALB/c mice were randomly divided into 3 groups.Animal models with Pseudomonas aeruginosa pneumonia were established.Low dose polymyxin B was used to neutralize the toxic and immunologic effects of endotoxin.Then the plasma endotoxin levels,interleukin-1 β mRNA levels,tumor necrosis factor α mRNA levels in lungs among different groups were compared.Results The plasma endotoxin levels of mice with Pseudomonas aeruginosa pneumonia were significantly higher than that with injection of Pseudomonas aeruginosa and low dose polymyxin B 24 hours postinfection(P 0.01).The interleukin-1β mRNA levels in lungs of mice with Pseudomonas aeruginosa pneumonia were significantly higher than that with injection of Pseudomonas aeruginosa and low dose polymyxin B 24 hours postinfection(P 0.01).The tumor necrosis factor α mRNA levels in lungs of mice with Pseudomonas aeruginosa pneumonia were significantly higher than that with injection of Pseu domonas aeruginosa and low dose polymyxin B 24 hours postinfection(P 0.01).There was a positive correlation between plasma endotoxin level and interleukin-1β or tumor necrosis factor α mRNA level in mice with Pseudomonas aeruginosa pneumonia(r=0.98,P 0.01;r=0.97,P 0.01).Conclusion Plasma endotoxin level correlated with interleukin-1β or tumor necrosis factor α mRNA level in mice with Pseudomonas aeruginosa pneumonia..
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