VDR基因多态性与宁夏回汉儿童哮喘差异性研究  

Differences study of asthma and polymorphisms in vitamin D receptor gene between Ningxia Hui and Han asthmatic children

作　　者：汤长超[1] 马金海[2] 张晓春[2] 张学红[2] 高英[2] 白华[2] 

机构地区：[1]宁夏医科大学总医院临床学院儿科学系,宁夏银川750004 [2]宁夏医科大学总医院儿科,宁夏银川750004

出　　处：《中国儿童保健杂志》2013年第7期691-694,共4页Chinese Journal of Child Health Care

基　　金：宁夏回族自治区自然科学基金自助项目(NZ10130)

摘　　要：【目的】探讨VDR基因BsmI、ApaI、TaqI和FokI位点的单核苷酸多态性在宁夏回汉族哮喘儿童之间的差异性。【方法】提取回汉族哮喘儿童外周血DNA各60例,采用PCR及PCR-RFLP方法分析VDR基因四个多态位点的基因型及基因频率,采用χ2检验分析相关数据。【结果】FokI位点是宁夏回汉族儿童哮喘之间存在差异性的一个位点(P<0.05)。BsmI、ApaI及TaqI位点在宁夏回汉族儿童哮喘之间差异无统计学意义(P>0.05)。【结论】在所研究的VDR基因的四个多态位点中:FokI位点在宁夏回汉族儿童哮喘之间存在差异性;其余位点与在宁夏回汉族儿童之间差异无统计学意义。【Obiective】 To discuss the differences between Ningxia Hui and Han asthmatic children about the single nucleotide polymorphisms of locus of BsmI,ApaI,TaqI and FokI on the VDR gene.【Methods】 The peripheral blood DNA from Hui and Han asthmatic children was extracted to analyze the genotype and gene frequency of dynamic locus of VDR gene using the methods of PCR and PCR-RFLP,Chi square test was used to analyse data.【Results】 FokI was a locus where the difference of asthma existed between Ningxia Hui and Han asthmatic children（P0.05）.There was no distinguished difference about the distribution of gene frequency and genotype frequency of the locus of BsmI,ApaI and TaqI existed between the Hui asthma group and Han asthma group（P0.05）.【Conclusions】 Among the four polymorphic sites being studied,the following results have been achieved：FokI is a locus where the differences exist between Ningxia Hui children＇s asthma and Ningxia Han children＇s asthma.There is no difference between Ningxia Hui and Han children＇s asthma about the locus of BsmI,ApaI and TaqI.

关 键 词：支气管哮喘 VDR基因 单核昔酸多态 儿童 

分 类 号：R725.6[医药卫生—儿科]