胎兔骨髓基质前成骨细胞的培养及鉴定  

Culture and Indentification of Osteogenic Cells in Fetal Rabbit Bone Marrow

作　　者：张银刚[1] 杨述华[1] 罗怀灿[1] 李进[1] 

机构地区：[1]同济医科大学附属协和医院骨科,武汉430022

出　　处：《同济医科大学学报》2000年第4期331-333,共3页Acta Universitatis Medicinae Tongji

基　　金：湖北省自然科学基金!资助项目 (No.99J2 30 )

摘　　要：为了证实体外培养的胎兔骨髓基质骨祖细胞具有成骨细胞系特征。采用胎兔长管骨骨髓细胞 ,加入 DMEM培养 ,传代四代后 ,用活体相差显微镜、透射电镜、组织化学染色、免疫组化等方法进行观测。结果显示体外培养的基质细胞具有成骨细胞系特征。提示胎兔骨髓基质骨祖细胞 ,可以作为修复骨缺损的种子细胞。In order to confirm the in vitro cultured fetal bone marrow osteogenic cells having osteogenic features, fetal rabbit long bone marrow cells were cultured in DMEM medium. After four generations for passage, the cells were observed under transmission electron microscopy, stereoscopic microscopy, and by using histochemical and histoimmunogical methods. The results showed that the cultured stromal cells in vitro possessed the features of the osteogenic cells, suggesting fetal rabbit bone marrow cells could be used to repair the bone defect.

关 键 词：胎兔 骨髓基质细胞 成骨细胞 培养 鉴定 

分 类 号：R329－33[医药卫生—人体解剖和组织胚胎学] R336[医药卫生—基础医学]