血清生长激素结合蛋白浓度和肝脏生长激素受体表达与肾病大鼠生长障碍的关系  

Serum growth hormone binding protein and liver growth hormone receptor in nephrotic rats with growth failure

作　　者：刘建华[1] 易著文[2] 张宝林[2] 

机构地区：[1]上海市儿童医院肾内科 [2]湖南医科大学附属第二医院儿科,长沙410011

出　　处：《中华肾脏病杂志》2000年第4期239-243,共5页Chinese Journal of Nephrology

基　　金：湖南省自然科学基金资助项目![湘科计字97(37-17)

摘　　要：目的　探讨营养不良、肾病本身和糖皮质激素作为各自独立因素对大鼠血清生长激素结合蛋白（ＧＨＢＰ）及肝脏生长激素受体（ＧＨＲ）表达的影响，阐明ＧＨＢＰ／ＧＨＲ改变与肾病综合征（ＮＳ）大鼠生长障碍的关系。方法　２４只周龄相同体重相近的雄性ＳＤ大鼠被随机分成正常、食物对照、阿霉素（５ｍｇ· ｋｇ）肾病和地塞米松（１．８ ｍｇ· ｋｇ－１· ｄ－１）治疗的阿霉素肾病４组。血清 ＧＨＢＰ、ＧＨＢＰ－１浓度及肝脏ＧＨＲ表达分别采用墨炭包被的葡聚糖蛋白分裂技术、凝胶层析和放射受体分析法检测。结果１．和正常及食物对照组大鼠相比，肾病和激素治疗组血清ＧＨＢＰ和肝脏ＧＨＲ的减低其差异有显著性意义，但食物对照组较正常组，激素治疗组较肾病组之减低，其差异无显著意义。２．血清ＧＨＢＰ－１浓度依正常、食物对照、肾病和激素治疗组顺序逐一减低。３．血清ＧＨＢＰ浓度及肝脏ＧＨＲ表达与大鼠鼻尾长度和体重均呈正相关（Ｐ均＜０．０１）。结论继发性营养不良和肾病本身所致的生长障碍与肝脏ＧＨＲ表达下降引发的生长激素抵抗有关，糖皮质激素治疗后生长激素抵抗加重也是其生长障碍加剧的重要因素。Objective To investigate the effects of malnutrition, nephrosis itself and glucocorticoid therapy on serum growth hormone binding protein(GHBP) and liver growth hormone receptor(GHR), and elucidate the relationship between growth failure in nephrotic rats and serum GHBP or liver GHR. Methods Twenty-four male Sprague-Dawley(SD) rats were randomly divided into control, pair-fed, doxoruhincin-induced nephrotic (nephrotic) and dexamethasone-treated nephrotic (des-treated ) rats. Serum GHB P, GHB P- 1 and liver GHR were measured by dextran-coated charcoal technique, gel filtration and radioreceptor assay respectively. Results (1) Serum GHBP and liver GHR were reduced in nephrotic and des-treated rats compared with control and pair-fed rats, but no significant difference was found between control and pair-fed rats or between nephrotic and des-treated rats. (2)Serum GHBP-1 was lower in pair-fed rats, even lower in nephrotic rats and lowest in des-treated rats than in control. (3) There was some significantly positive correlations between nose-tail length or weight and serum GHBP or liver GHR. Conclusion GH resistance, due to decreasd liver GHR, is an important cause of growth failure induced by secondary malnutrition and nephrosis itself Glucocorticoid therapy deteriorates growth failure by further decreased hepatic GHR.

关 键 词：肾病综合征 生长激素结合蛋白 生长障碍 GHR 

分 类 号：R726.9[医药卫生—儿科]