淫羊藿苷和淫羊藿素对乳腺癌T47D细胞增殖的影响  

Effects of icariin and icaritin on proliferation of breast cancer T47D cells

作　　者：王洁[1,2] 陈花[2] 买迪娜[2] 马海蓉[2] 王振华[1] 李一[1] 

机构地区：[1]石河子大学药学院新疆特种植物药资源教育部重点实验室,新疆石河子832002 [2]中国科学院新疆理化技术研究所干旱地区植物资源化学重点实验室,新疆乌鲁木齐830011

出　　处：《中草药》2013年第11期1470-1475,共6页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81102890;11175222);教育部新世纪优秀人才支持计划(NCET-10-0967)

摘　　要：目的研究淫羊藿苷及淫羊藿素对乳腺癌T47D细胞增殖的影响,探讨雌激素受体(ER)与其作用机制的关系。方法用不同浓度的淫羊藿苷和淫羊藿素处理雌激素受体呈阳性的乳腺癌T47D细胞系后,MTT法检测T47D细胞增殖,流式细胞术检测T47D细胞周期的变化,Western blotting法检测T47D细胞ERα和ERβ蛋白表达。结果淫羊藿苷和淫羊藿素浓度为1×10 9～1×10 6mol/L,能显著促进T47D细胞增殖,且该作用可被雌激素受体拮抗剂ICI 182.780(1×10 6mol/L)所拮抗。与对照组相比,淫羊藿苷和淫羊藿素1×10 7mol/L使S期T47D细胞比例明显增加,增殖指数显著升高,但该作用可被ICI 182.780抑制。淫羊藿苷和淫羊藿素1×10 7mol/L还可显著增加ERα、ERβ蛋白表达(P<0.01)。结论淫羊藿苷和淫羊藿素均可显著促进T47D细胞增殖,该作用可能是通过上调胞内ERα、ERβ蛋白表达介导的。Objective To investigate the effects of icariin （Ica） and icaritin Oct） on the proliferationin of breast cancer T47D cells in vitro and to explore the relationship between estrogen receptor （ER） and the mechanisms. Methods The ER positive T47D cells were treated with Ica and Ict at different concentration. The proliferation of T47D cells was measured by MTT method, and the cell cycle changes during the proliferation were further evaluated using flow cytometric assay. The effects of Ica and Ict on the expression of ERα and ERβ in T47D cells were analyzed by Western blotting. Results Ica and Ict （10-9-10-6 mol/L） stimulated the proliferation of T47D cells, and the proliferation was inhibited by the estrogen antagonist ICI 182.780 00-6 tool/L）. Compared with the control group, Ica and Iα （10-7 tool/L） could increase the amount of T47D cells arrested in S phase, and the stimulation was inhibited by ICI 182.780. As well Ica and Ict （10-7 tool/L） could up-regulate the expression of ERct and ERβ proteins （P 〈 0.01）. Conclusion Ica and Ict stimulate the proliferation of T47D cells, and the effect may be performed through up-regulating the expression of ERa and ERβ proteins.

关 键 词：淫羊藿苷 淫羊藿素 乳腺癌T47D细胞 细胞增殖 雌激素受体 

分 类 号：R282.710.5[医药卫生—中药学] R979.19[医药卫生—中医学]