淫羊藿甙抗癌作用机制的实验研究  

Experimental Studies of Icariin on Anticancer Mechanism

作　　者：毛海婷[1] 张玲[1] 王芸[1] 李晓冰[1] 

机构地区：[1]山东省医学科学院基础所,济南250062

出　　处：《中药材》2000年第9期554-556,共3页Journal of Chinese Medicinal Materials

基　　金：山东省自然科学基金(Y96C14046)

摘　　要：中药单体淫羊藿甙是一种新型的生物反应调节剂和诱导分化剂。为进一步探讨淫羊藿甙抗癌作用机制,我们将淫羊藿甙作用于人高转移肺癌细胞PG,采用MTT、放免、流式细胞术。粘附实验。运动侵袭实验等多种手段进行了检测。结果表明,淫羊藿甙虽直接抑癌作用较弱,但却能影响PG细胞周期的时相分布,使S期减小,并能升高细胞内cAMP水平,降低cGMP水平,提高cAMP/cGMP比值,同时降低PG细胞对胞外基质的粘附性及侵袭、运动能力,从而逆转肿瘤细胞的恶性表型。提示,淫羊藿甙作为一种有开发前景的抗肿瘤药物值得进一步深入研究。Icariin(ICA)is a kind of new biological respone modifier(BRM)and differentiational agent. In order to further elucidate the reversion of malignant phenotypes of tumor cells and the mechanism of its action, highly metastatic human lung cancer cells(PG)were treated with ICA in vitro.In this study, MTT assay, radioimmune assay, flow cytometry( FCM) and invasion assay were used. The results showed that ICA could influence the distribution of PG cells cycle and reduce S phase. Moreover, ICA increased the level of cAMP in PG cells, reduced the level of cGMP and increased the cAMP/cGMP ratio.On the other hand, ICA decreased PG cells adhesive ratio to laminin substrate and decreased the ability of invasion or migration.These data demonstrate that ICA maybe a kind of effective anticancer drug.

关 键 词：淫羊藿甙 PG细胞 细胞周期 实验研究 抗癌作用 

分 类 号：R285.5[医药卫生—中药学]