RP-HPLC测定多叶棘豆全草中2个黄酮苷含量  

Content determination of two flavonoid glycosides in the whole herbs of Oxytropis myriophylla by RP-HPLC

作　　者：刁玉林[1] 王思[1] 折改梅[1] 刘慧[1] 刘斌[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《药物分析杂志》2013年第6期1026-1028,1040,共4页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立多叶棘豆全草中2个黄酮苷类化合物异鼠李素-3-O-α-L-吡喃鼠李糖基-(1→2)[O-α-L-吡喃阿拉伯糖基-(1→6)]-O-β-D-吡喃葡萄糖苷(WS-3)和异鼠李素-3-O-α-L-吡喃阿拉伯糖基-(1→6)-O-β-D-吡喃葡萄糖苷(WS-16)的含量测定方法。方法:采用RP-HPLC方法,使用SunFire C18色谱柱(150 mm×4.6 mm,5μm),以乙腈-0.1%甲酸水(17∶83)为流动相,流速1.0 mL.min-1,检测波长350 nm,柱温30℃。结果:WS-3和WS-16的线性范围分别为0.045～0.226μg(r=0.9998,n=5)和0.042～0.21μg(r=0.9997,n=5);平均回收率(n=6)分别为101.1%(RSD=1.6%)和100.5%(RSD=2.2%)。结论:适用于多叶棘豆药材的质量控制和评价。Objective： To establish a method for the content determination of two flavonoid glycosides,isorhamnetin - 3 - O - α - L - rhamnopyranosyl - （ 1→2 ） [ O - α - L - arabinopyranosyl - （ 1 →6 ） ] - O -β - O - glueopyrano- side （ WS - 3 ） and isorhamnetin - 3 - O - α - L - arabinopyranosyl - （ 1→6） - O -β - D - glucopyranoside （ WS - 16）, in the whole herbs of Oxytropis myriophylla （Pall.） DC.. Methods：The RP - HPLC separation was performed on a SunFire C18 analytical column（ 150 mm ×4.6 mm,5μm）,and the mobile phase was acetonitrile-water con-taining 0. 1% formic acid（ 17：83 ） at a flow rate of 1.0 mL · min^-1. The detection wavelength was set at 350 nm and the column temperature was 30 ℃. Results：The linear ranges of WS-3 and WS - 16 were 0. 045-0. 226 μg （ r = 0. 9998, n = 5 ） and 0. 042 - 0. 21 μg （ r = 0. 9997, n = 5 ）, respectively. The average recoveries （ n = 6 ） were 101.1% （RSD = 1.6% ） and 100. 5% （RSD =2. 2% ） ,respectively. Conclusion：The method can be used for qual-ity control and evaluation of the O. myriophyUa herbs.

关 键 词：多叶棘豆全草 黄酮苷 异鼠李素-3-O-α-L-吡喃鼠李糖基-(1→2)[O-α-L-吡喃阿拉伯糖基-(1→6)]-O-β-D-吡喃葡萄糖苷 异鼠李素-3-O-α-L-吡喃阿拉伯糖基-(1→6)-O-β-D-吡喃葡萄糖苷 含量测定 反相高效液相色潽法 

分 类 号：R917[医药卫生—药物分析学]