棉花GhTCP2基因启动子分离及其在幼嫩组织的表达分析  

Isolation of the Cotton GhTCP2 Gene Promoter and Analysis of its Expression in Young Tissues

作　　者：邢进[1,2] 刘伟娜[1] 赵琳琳[3] 赵金凤[1] 张文会[3] 赵宝华[2] 李学勇[1] 

机构地区：[1]中国农业科学院作物科学研究所/农作物基因资源与基因改良国家重大科学工程,北京100081 [2]河北师范大学生命科学学院,河北石家庄050024 [3]聊城大学生命科学学院,山东聊城252059

出　　处：《棉花学报》2013年第3期197-204,共8页Cotton Science

基　　金：国家自然科学基金(31071078)

摘　　要：采用Genome walking的方法分离了GhTCP2基因的启动子区,应用在线分析软件PLACE对该启动子序列进行分析,发现含有多个与细胞周期、细胞分裂素、生长素相关的顺式作用元件。构建了GhTCP2基因启动子驱动报告基因GUS的植物表达载体并转化了模式植物拟南芥。转基因拟南芥的组织化学染色显示,GUS基因主要在根尖、幼叶、顶芽、侧芽和花蕾中表达。这说明棉花GhTCP2基因启动子可以驱动目的基因在植物的幼嫩组织表达,该启动子有望在植物抗虫基因工程中应用。Expression of the cotton GhTCP2 gene, a member of the TCP family, was detected by RT-PCR in young tissues, such as apical, lateral, and floral buds, of cotton plants. To study GhTCP2 gene expression patterns in detail, the GhTCP2 promoter region was isolated using the genome walking technique. Many c/s-regulatory motifs related to the cell cycle, cytokinin, and auxin were identified using the on-line software program PLACE. The GhTCP2 promoter was inserted upstream of the GUS reporter gene, and the resulting plant expression construct was transformed into Arabidopsis. Histological staining of transgenic Arabidopsis plants revealed that the GhTCP2 promoter-driven GUS gene was expressed in root tips, young leaves, and apical, lateral, and floral buds. This result indicates that the cotton GhTCP2 promoter is able to drive foreign gene expression in young plant tissues. The GhTCP2 promoter has the potential to be utilized in plant insect-resistance genetic engineering to protect young plant tissue fi＇om insect attack.

关 键 词：棉花 TCP家族转录因子 启动子 特异性表达 

分 类 号：S562[农业科学—作物学]