EE2与DBP对斑马鱼胚胎发育的联合毒性研究  

Joint Toxicity of EE2 and DBP to Embryonic Development of Zebrafish(Brachydanio rerio)

作　　者：陈朋宇[1] 刘磊[1] 李松[2] 许楠[1] 徐一峰[1] 范重阳[1] 

机构地区：[1]北京大学深圳研究生院环境与能源学院城市人居环境科学与技术重点实验室,广东深圳518055 [2]北京大学深圳研究生院化学生物学与生物技术学院化学基因组学实验室,广东深圳518055

出　　处：《农业环境科学学报》2013年第6期1099-1105,共7页Journal of Agro-Environment Science

基　　金：深圳市科技计划项目(CXB201105100085A)

摘　　要：基于斑马鱼胚胎技术和等毒性配比法,研究了乙炔雌二醇(EE2)与邻苯二甲酸二丁酯(DBP)对斑马鱼的联合胚胎毒性,主要观察并比较了斑马鱼96h内胚胎发育情况、死亡率和孵化率,并分别采用相加指数法、毒性单位分析法和毒性单位图解法分析比较了EE2和DBP组合在不同时间和浓度对斑马鱼胚胎发育的效应类型。结果表明:斑马鱼的胚胎发育对EE2和DBP的毒性作用较为敏感,均表现出显著的浓度依赖性和时间的累加效应,可以造成胚胎发育畸形(尾畸、水肿、脊柱弯曲、孵化延迟等)甚至死亡;DBP的毒性作用高于EE2,DBP和EE2的96h-LC50分别为2.219mg·L-1和3.975mg·L-1;EE2和DBP组合在不同时间和浓度有不同的效应类型,在80h时二者之间表现出拮抗作用,96h时则表现出协同作用。In studying the joint toxicity of ethynylestradiol（EE2） and dibutyl phthalate（DBP） on the embryonic development of zebrafish, a 1∶1 ratio of EE2 and DBP was used. The primary research objective was to observe and compare the embryonic development, mortality, and hatchability behaviors of zebrafish embryos within a 96-hour time frame. Furthermore, using a trifold technique of additive index method, toxic unit analytical method, and toxicity unit graphic method, we analyzed the effects of EE2 and DBP on zebrafish in varying doses and under varying time conditions. The results illustrated that zebrafish embryos were sensitive to the toxic effects of EE2 and DBP, thus demonstrating a significant sensitivity to both concentration of the dose and accumulation of time. The embryos begun to develop defects as well（e.g., tail defects, edema, spine curvature, delayed hatching）. The toxicity of DBP was higher than that of EE2; the 96-hour median lethal concentration（LC50） of DBP and EE2 were 2.219 mg·L^-1 and 3.975 mg·L^-1, respectively. The joint-dose of DBP and EE2 produced an antagonistic effect on mortality of zebrafish at 80 hours but a synergistic effect at 96 hours.

关 键 词：乙炔雌二醇(EE2) 邻苯二甲酸二丁酯(DBP) 斑马鱼 胚胎 毒性 

分 类 号：X503.225[环境科学与工程—环境工程]