超声激励微泡增强VX2移植瘤细胞膜通透性的实验研究  

Study on Enhancing Cellular Membrane Permeability of VX2 Xenografted Tumor by Ultrasound-activated Microbubbles

作　　者：许婷婷[1] 吴凤林[1] 罗利红[1] 胡洁[1] 隋洋[1] 

机构地区：[1]南方医科大学南方医院超声科,广州市510515

出　　处：《中国超声医学杂志》2013年第6期568-571,共4页Chinese Journal of Ultrasound in Medicine

基　　金：广东省自然科学基金(No.10151051501000048)

摘　　要：目的探讨超声激励微泡增强VX2移植瘤细胞膜通透性。方法建立兔VX2皮下移植瘤模型,按超声强度随机分为0、0.21、0.43、0.86、1.09 W/cm^2 5组,辐照时间10 min;同时经耳缘静脉缓慢注射"全氟显"微泡造影剂0.12 ml/kg,至辐照完毕,即刻切取瘤组织行透射电镜硝酸镧示踪观察胞浆内镧颗粒的数量。结果 VX2移植瘤细胞浆内镧颗粒随着超声辐照强度增加而增多,当0.43 W/cm^2时,胞浆内镧颗粒最多;之后随强度进一步提高,镧颗粒则减少。结论超声激励微泡能够提高VX2移植瘤细胞膜的通透性。Objective To investigate the effects of ultrasound activated protein microbubbles with different inten sity on the cellular membrane permeability of VX2 xenografted tumor. Methods 25 VX2 carcinoma bearing New Zeal- ane white rabbits were randomized into 5 groups with 5 rabbits in each group. Ultrasound with the pulse duration of 4.84 ms, and the intensity of 0,0.21,0.43,0.86,1.09 W/cm^2 was applied on the VX2 xenografted tumors of 5 groups respectively. Microbubbles under the dosage of 0.12 ml/kg were administrated simultaneously via ear marginal vein throughout the ultrasonic irradiation. After being continuously exposed for 10 min, xenografted tumors were harvested immediately for tansmission electron microscope by means of Lanthanum（La） tracer to observe the alteration of cellu lar membrane permeability. Results In a certain range, when pulsed duration, irradiation time and microbubble con centration remained as unchanged, number of La in cytoplasm of VX2 xenografted tumor may be enhanced with in creased intensity of ultrasound. However, if the ultrasound intensity exceeded over 0.43 W/cm^2 , number of La de creased dramatically. Conclusions Ultrasound activated microbubbles could increase the cellular membrane permeabil- ity of VX2 xenografted tumor.

关 键 词：超声 微泡 通透性 镧颗粒 

分 类 号：R445.1[医药卫生—影像医学与核医学] R73-36[医药卫生—诊断学]