日本血吸虫病患者尿液中循环抗原和抗体联合检测的诊断价值  

THE DIAGNOSTIC VALUE OF COMBINED DETECTION OF CIRCULATING ANTIGENS AND ANTIBODIES IN URINE OF PATIENTS WITH SCHISTOSOMIASIS JAPONICA

作　　者：张恩英[1] 娄文娴[1] 薛纯良[1] 王兆军 

机构地区：[1]上海第二医科大学寄生虫学教研室,200025

出　　处：《中国寄生虫学与寄生虫病杂志》2000年第1期24-25,共2页Chinese Journal of Parasitology and Parasitic Diseases

基　　金："九 .五"国家重点基金!资助课题!(No 0 4 0 2 0 2 );国家自然科学基金!资助课题 (No 39570 644);上海市科委自然科学基金!资助课

摘　　要：[目的 ]探讨尿液中血吸虫循环抗原和抗体检测对日本血吸虫病的诊断价值。 [方法 ]用单克隆抗体夹心 ELISA法检测日本血吸虫病患者尿液中循环抗原 ,间接ELISA检测尿液中特异性抗体。 [结果 ]10例急性血吸虫病和 6 1例慢性血吸虫病患者尿液中循环抗原的阳性率分别为 6 0 %和 40 % ,特异性抗体的阳性率分别为 80 %和 6 1 7%。两者联合检测的总阳性率分别为 10 0 %和 71 7%。 10 0例健康对照者尿液中仅 3%出现假阳性。 [结论 ]检测尿液中日本血吸虫循环抗原和特异性抗体简便、实用 ,为一种非损伤性的血吸虫病诊断方法。Objective] To assess the value of detecting circulating antigens and antibodies in urine as a noninvasive method for the diagnosis of schistosomiasis. [Methods] A sandwich ELISA and ELISA using McAb were applied to detect circulating schistosomal antigens and specific antibodies in the urine of patients with acute and chronic schistosomiasis. [Results] When the urine samples from 10 cases of acute schistosomiasis and 61 cases of chronic schistosomiasis were examined, the positive rates of circulating antigens and specific antibodies were 60%,40%, and 80%, 60 1%, respectively; when both detection was combined, the positive rates were 100% and 71 7% respectively, whereas a false positive rate of 3% of CAg or CAb was detected in the urine of 100 normal controls. [Conclusion] The detection of circulating antigen in urine is a practicable and noninvasive method for the diagnosis of schistosomiasis.

关 键 词：日本血吸虫病 循环抗原 特异性抗体 诊断 

分 类 号：R532.210.4[医药卫生—内科学]