SPA-ELISA检测弓形虫感染鼠尿中的循环抗原  

DETECTON OF CIRCULATING ANTIGEN IN URINE FROM MICE INFECTED WITH TOXOPLASMA TACHYZOITES BY SPA-ELISA

作　　者：金武官[1] 李云珠[1] 俞善昌[1] 杨惠珍[2] 

机构地区：[1]上海第二医科大学附属瑞金医院儿科,上海200025 [2]上海第二医科大学寄生虫教研组,上海200025

出　　处：《中国寄生虫学与寄生虫病杂志》2000年第1期43-45,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家自然科学基金!资助项目 (No . 390 70 754)

摘　　要：[目的 ]探索弓形虫感染早期诊断的简便方法。 [方法 ]用SPA ELISA检测轻度、中度和重度感染弓形虫鼠尿中弓形虫循环抗原 (TCA)。 [结果 ]轻度、中度和重度感染弓形虫 3组鼠尿中均检出TCA ,上述 3组分别于弓形虫感染后d6 、d3和d4显示阳性斑点。 [结论 ]SPA ELISA能检出弓形虫感染鼠尿中的弓形虫循环抗原 ,可用于弓形虫感染的早期诊断。Objective] To explore a simple and convenient immunoassay for early diagnosis of Toxoplasma infection.[Methods] Urine samples collected from three groups of mice infected with different doses of tachyzoites were detected for Toxoplasma circulating antigen (TCA) by dot ELISA using HRP SPA as a second antibody ( SPA ELISA).[Results] Toxoplasma circulating antigens were detected in all three groups of infected mice in contrast with the normal control group. Toxoplasma circulating antigen was detected on d 6 and d 3 after infection in mice of light and moderate infection groups, respectively.[Conclusion]SPA ELISA is a simple and convenient method for early immunodiagnosis of recent Toxoplasma infection.

关 键 词：循环抗原 SPA-ELISA 弓形体病 IGG 制备 

分 类 号：R531.804[医药卫生—内科学]