实验性大鼠脑动脉瘤形成过程中MMP-2、MMP-9表达的动态变化  

Expression of matrix metalloproteinase 2 and 9 in hypertension-induced intracranial aneurysm in rats

作　　者：马良[1] 付强[1] 关俊宏[1] 

机构地区：[1]中国医科大学附属盛京医院神经外科,辽宁沈阳110004

出　　处：《中华神经外科疾病研究杂志》2013年第3期197-200,共4页Chinese Journal of Neurosurgical Disease Research

摘　　要：目的探讨实验性大鼠动脉瘤形成过程中基质金属蛋白酶2(MMP-2)、基质金属蛋白酶9(MMP-9)的表达规律。方法制作肾性高血压大鼠脑动脉瘤模型,通过免疫组化在蛋白水平系统动态观察肾性高血压大鼠脑动脉瘤形成过程中MMP-2、MMP-9表达的变化。结果实验组在术后1 w脑动脉壁即可见MMP-2、MMP-9表达增加,随着术后时间的推移和大鼠血压的增高,其表达也迅速增加,术后1个月基本达最高峰并一直持续至4个月,其中MMP-9较正常状态的增加比MMP-2的表达增加更为显著。对照组脑动脉壁MMP-2、MMP-9也有微弱表达,且MMP-2表达较MMP-9略强。结论脑动脉壁MMP-9、MMP-2特别是MMP-9的过度表达导致的脑动脉壁胶原纤维及内弹力层破坏是脑动脉瘤形成的主要原因之一。Objective To investigate the expression pattern of matrix metalloproteinase-2 （MMP-2）, matrix metaUoproteinase-9 （MMP-9） in the process of aneurysm formation in rats. 1Vleflaods A rat model of cerebral anettrysm was established. The dynamic changes of MMP-2 and MMP-9 expressions were examined by immtmohistochemistry at the level of protein activity in the process of aneurysm formation. Results In experimental group, with the development of hypertension, the expressions of MMP-2 and MMP-9 were increased one week after operation, peaked at first month and lasted until 4 months after operation. The expression of MMP-9 was increased more remarkably than that of MMP-2. Weak staining of MMP-2 and MMP-9 was observed in the cerebral artery, and the expression of MMP-2 was little higher than that of MMP-9. Conclusion The over-expressions of MMP-2 and MMP-9 in artery which result in the wall destruction of collagen and internal elastic lamina of cerebral artery are the main cause of the aneurysm formation.

关 键 词：颅内动脉瘤 基质金属蛋白酶2 基质金属蛋白酶9 

分 类 号：R651.1[医药卫生—外科学]