β-catenin和WISP-1在糖尿病大鼠心肌中的表达  

Expressions of myocardial β-catenin and WISP-1 in diabetic rats

作　　者：汪华学[1] 陶静[2] 叶红伟[2] 冯振中[3] 高琴[2] 

机构地区：[1]蚌埠医学院第一附属医院重症监护中心,安徽233004 [2]蚌埠医学院生理学教研室,安徽233030 [3]蚌埠医学院病理学教研室,安徽233030

出　　处：《中国组织化学与细胞化学杂志》2013年第3期189-192,共4页Chinese Journal of Histochemistry and Cytochemistry

基　　金：国家自然科学基金资助(81000074);安徽省教育厅科研项目资助(KJ2012Z254)

摘　　要：目的观察Wnt信号通路中β-catenin及其下游靶基因WISP-1在糖尿病大鼠心肌组织中的表达,分析Wnt/β-catenin信号通路在糖尿病大鼠心肌损伤中的作用。方法雄性SD大鼠随机分为正常对照组(Control Group)和糖尿病模型组(DM Group),腹腔注射STZ 55mg/kg诱导糖尿病大鼠模型,喂养至8周。测定大鼠的空腹血糖、心体比和心肌羟脯氨酸含量;电镜观察心肌超微结构变化,免疫组化法观察心肌组织β-catenin和WISP-1的表达。结果与对照组相比,糖尿病组大鼠空腹血糖水平明显增加,心体比增加,心肌羟脯氨酸含量增高。心肌超微结构显示肌纤维断裂,线粒体呈空泡样改变。心肌β-catenin和WISP-1蛋白表达增加。结论糖尿病大鼠心肌组织β-catenin和其下游靶基因WISP-1表达增加,提示Wnt/β-catenin信号通路的激活参与糖尿病所致的心肌损伤。Objective To observe the expressions of myocardial-catenin and Wnt-induced secreted protein 1 （WISP-1） in diabetic rat, and analyze the role of Wnt/β-catenin signaling pathway in diabetes-in- duced myocardial injury. Methods Male SD rats were divided into control group and diabetes group. The diabetic rats were injected with STZ at 55mg/kg intraperitoneally and fed to 8th week. The plasma fasting blood glucose （FBG） level, heart weight/body weight （HW/BW） and myocardial hydroxyproline （Hp） content were measured. Myocardium ultrastructure was observed by electron microscopy. The expressions of myocardial β-catenin and WISP-1 protein were measured by immunohistochemical staining. Results In contrast to those of the control group, FBG level, HW/BW and myocardial Hp content were increased in the diabetes group. The breakage of myofibrils and mitochondria injury were aggravated. The expressions of myocardial t3 catenin and WISP-1 were higher than in the control group. Conclusion The protein expres- sions of myocardial β catenin and the downstream gene WISP-1 were increased in diabetic rat, which suggests that the activation of the Wnt/β-catenin signaling pathway participated in diabetes-induced myocardial injury.

关 键 词：糖尿病 Β-CATENIN WISP-1 WNT信号通路 心肌损伤 

分 类 号：R322.11[医药卫生—人体解剖和组织胚胎学]