冠心病患者血浆循环游离线粒体DNA定量分析及其临床意义  

Quantitative analysis of circulating freeing mitochondrial DNA in plasma of patients with coronary heart disease and its potential clinical value

作　　者：谢晓莉 王玉静 宋诗芹[2] 李红梅 高延 余福林 

机构地区：[1]解放军323医院心内科,陕西西安710054 [2]甘肃省军区门诊部

出　　处：《西北国防医学杂志》2013年第3期233-235,共3页Medical Journal of National Defending Forces in Northwest China

摘　　要：目的:检测冠心病(CHD)患者外周血血浆循环游离线粒体DNA(ccf-mtDNA)含量并初步探讨其临床应用价值。方法:收集106例CHD患者和106例健康志愿者的外周血样本,以荧光实时定量PCR方法检测各组血浆ccf-mtDNA含量,比较分析血浆ccf-mtDNA水平与CHD的临床相关性。结果:CHD患者血浆ccf-mtDNA含量明显高于对照组(P<0.01)。以CHD组血浆ccf-mtDNA含量绘制ROC曲线,曲线下面积为0.724(95%可信区间为0.665～0.783),以血浆ccf-mtDNA含量≥0.71×106为临界值,用ccf-mtDNA诊断CHD的敏感度为74.7%,特异度为73.0%。血浆ccf-mtDNA含量与CHD患者SBP、DBP、TC、LDL-C、FPG显著正相关(P<0.05),与年龄、HDL-C显著负相关(P<0.05)。结论:血浆ccf-mtDNA含量对CHD患者的早期诊断及病程监测具有重要的临床价值,可作为CHD事件发生的监测指标。Objective： To detect the level of the circulating freeing mitochondrial DNA （ ccf - mtDNA） in plasma of patients with coronary heart disease （CHD） and to evaluate its potential clinical application values. Methods： Peripheral blood samples were collected from 106 cases of CHD and 106 cases of healthy controls. The mtDNA con-tent in the peripheral blood was detected by real - time fluorescence quantitative PCR assay, the associativity of ccf - mtDNA content and clinical characteristic of CHD was compared and analyzed. Results： The level of ccf - mtD-NA in CHD group was significantly higher than that of healthy controls （P 〈 0.01 ）. ROC curve was drown accord- ing to the eef - mtDNA level, area under curve was 0.724 （95% CI： 0. 665 - 0.783 ）. The level of cef - mtDNA ≥0.71 × 10^6 was used as critical value to diagnose CHD, the sensitivity and specificity was 74.7% and 73.0%, respectively. Plasma ccf-mtDNA content was positively associated with SBP, DBP, TC, LDL - C, and FPG, but negatively associated with age and HDL - C （ P 〈 0.05 ）. Conclusion： Plasma ccf - mtDNA content play an impor-tant role in early diagnosis of CHD. It can be used as a monitoring indicator for CHD.

关 键 词：冠心病 线粒体DNA 聚合酶链反应 发病风险 

分 类 号：R541.4[医药卫生—心血管疾病]