抑癌基因PDLIM4在前列腺癌细胞中的差异表达  

Differential expression and regulation of PDLIM4 in prostate cancer cells

作　　者：李先哲[1] 司曼飞[1,2] 郭艳霞[1] 苑辉卿[1] 

机构地区：[1]山东大学医学院生物化学与分子生物学研究所,济南250012 [2]山东大学医学院临床医学2009级五年制,济南250012

出　　处：《山东大学学报（医学版）》2013年第6期34-39,共6页Journal of Shandong University:Health Sciences

摘　　要：目的探讨PDLIM4基因在正常前列腺上皮细胞(RWPE1)、前列腺癌细胞(PC-3、DU145、LNCaP)、前列腺癌多药耐药细胞(PC-3/Doc)及其他耐药细胞(K562/A02、H460/Doc)中的差异表达。方法采用定量PCR(qPCR)、Western blot、免疫荧光等方法检测PDLIM4基因在各种细胞株中的表达;利用Western blo和DNA甲基转移酶(DNMTs)活性检测分析细胞中DNMTs的表达及活性。结果 PDLIM4基因在RWPE1高表达,在DU145、LNCaP细胞中基本无表达,在前列腺癌PC-3细胞中表达甚低,而在与其对应的多药耐药PC-3/Doc细胞中高表达(P<0.05),并且在耐药细胞株中的高表达具有组织特异性。DNMTs在RWPE1表达水平低于前列腺癌细胞,但在PC-3和PC-3/Doc中无差异。酶活性分析表明,PC-3/Doc中DNMTs的活性低于PC-3细胞(P<0.05)。结论 PDLIM4基因在前列腺癌细胞中低表达,这种特异性的在前列腺癌耐药细胞中表达显著上调可能是由DNMTs活性降低所致。Objective To analyze the differential expression of tumor suppressor PDLIM4 gene in nonneoplastic RWPE1 cells and prostate cancer （PC） cell lines and different drug-resistant cell lines. Methods Different cell lines including RWPE1, carcinoma cell lines （PC-3, DU145, and LNCaP）, multidrug-resistant cells （PC-3/Doc, K562/A02, H460/Doc） were used to identify PDLIM4 expression by quantitative PCR （qPCR）, Western blotting, and immunofluorescence （IF） assays. The expressions and activities of DNA methyltransferases （DNMTs） were also tested in PC-3 and PC-3/Doc cells by Western blotting and enzyme activity assays. Results PDLIM4 was overexpressed in RWPE1 cells, not expressed in DU145 or LNCaP cells, slightly detected in PC-3 cells but predominantly evidenced in its multidrug resistant PC-3/Doc cells, and this kind of high expression of PDLIM4 in drug-resistant cell lines is tissuespecific. Enzyme activity assays demonstrated that the activity of DNMTs were significantly reduced in PC-3/Doc compared to PC-3 cells, even though the expression levels of DNMTs were similar in both cell lines. Conclusion The expressions of PDLIM4 were dramatically reduced in PC cell lines, while this tissue-specific up-regulation of PDLIM4 in PC-3/Doc might result from the decreased enzyme activity of DNMTs when compared to PC-3 cells.

关 键 词：基因 PDLIM4 5-氮杂-2’-脱氧胞苷 多西紫杉醇 前列腺肿瘤 

分 类 号：R349.6[医药卫生—基础医学]