间歇性缺氧大鼠模型的建立及评价  

Establishment and evaluation of an intermittent hypoxia animal model in the rat

作　　者：闫静[1] 侯瑾[1] 盛颖[1] 王波涛[1] 祝康[1] 康全清[1] 

机构地区：[1]西安交通大学医学院第二附属医院耳鼻咽喉头颈外科病院,西安710004

出　　处：《山东大学学报（医学版）》2013年第6期53-56,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(30772404)

摘　　要：目的设计制造自动控制间歇性缺氧试验箱,用于儿童阻塞性睡眠呼吸暂停低通气综合征(OSAHS)间歇性缺氧动物模型的建立及评价。方法将24只SD大鼠随机分为3组:对照组、轻度缺氧组和重度缺氧组。后两组分别饲养于间歇缺氧箱内,通过调整参数,使大鼠在3个舱中直线往返运行,以实现动物间歇性缺氧。采用夹指型血氧饱和度仪检测不同低氧条件下血氧饱和度,进而模拟符合儿童OSAHS的缺氧条件。结果经过反复实验,当低氧舱中氧浓度为6%～8%时,轻度组在低氧舱停留8 s,过渡舱2 s,动物舱停留600 s,为1个周期,每天持续循环8 h,最后在过渡舱内测得平均血氧饱和度为(85.25±1.75)%。重度缺氧组在低氧舱停留8 s,过渡舱2 s,动物舱停留150 s,为1个周期,每天持续8 h,最后在过渡舱测定平均血氧饱和度为(77.63±3.78)%,轻、重度缺氧组大鼠返回动物舱时的血氧饱和度均为98%～100%,均实现了间歇性缺氧。轻度缺氧组睡眠呼吸暂停/低通气指数(AHI)为6次/h;重度缺氧组AHI为24次/h。缺氧程度、发生缺氧事件的时间及频率均符合儿童OSAHS轻、重度标准。结论自行研制的自动控制缺氧试验箱可用于建立儿童OSAHS间歇性缺氧动物模型。Objective To design and make an automatic control hypoxic chamber for the development and evaluation of the intermittent hypoxia animal model of children obstructive sleep apnea hypopnea syndrome（OSAHS）. Methods Twenty-four SD rats were randomly divided into 3 groups： control group, mild hypoxia group and severe hypoxia group. The latter two groups were raised in the intermittent hypoxic chamber. By adjusting the parameters, the rats in mild hypoxia group and severe hypoxia group were moved to and fro straightly among the 3 carbins of the automatic control hypoxic chamber in order to achieve intermittent hypoxia. Finger type blood oxygen saturation sensor was used to measure the oxygen saturation in different hypoxia conditions. Results When oxygen concentration in the hypoxic carbin reached 6%-8%, the rats in mild group stayed in the hypoxic carbin, transition cabin and animal cabin for 8 s, 2 s and 600 s respectively. After the above cycle was continuously repeated in 8 hours per day, average oxygen saturation of the rats in this group determined in the transition cabin reached （85.25±1.75）%. While, the rats in severe group stayed in the hypoxic carbin, transition cabin and animal cabin for 8 s, 2 s and 150 s respectively. After the above cycle was continuously repeated in 8 hours per day, average oxygen saturation of the rats in this group determined in the transition cabin reached （77.63±3.78）%. When the rats of the two groups returned into the animal cabin, their oxygen saturation were all between 98%-100%. Apnea-hypopnea index（AHI）in mild group was 6 times/h, and AHI in severe group was 24 times/h. Oxygen saturation, duration and frequency of hypoxic events were consistent with the mild and severe standard of children OSAHS. Conclusion The self-developed automatic control hypoxic chamber can be used to establish animal models of intermittent hypoxia of children OSAHS.

关 键 词：阻塞性睡眠呼吸暂停低通气综合征 自动控制 间歇性缺氧 儿童 动物模型 大鼠 Sprague—Dawley 

分 类 号：R766[医药卫生—耳鼻咽喉科]