重金属Cu^(2+)、Pb^(2+)单因子及联合毒性对泥鳅卵细胞DNA的损伤效应  

EFFECTS OF Cu^(2+) AND Pb^(2+)(SINGLE FACTOR AND JOINT TOXICITY) ON DNA DAMAGE IN MISGURNUS ANGUILLICAUDATUS OOCYTES

作　　者：唐建勋[1] 唐奕扬[2] 孙红祥[3] 李君荣[1] 吴瑗[1] 赵华[1] 

机构地区：[1]金华职业技术学院农业与生物工程学院,金华321007 [2]浙江交通技师学院,金华321015 [3]浙江大学动物科学学院,杭州310029

出　　处：《水生生物学报》2013年第3期501-506,共6页Acta Hydrobiologica Sinica

基　　金：浙江省自然科学基金项目(LY12C03006)资助

摘　　要：采用室内暴露试验方法,研究了不同浓度Cu2+(0.01、0.10、0.25mg/L)、Pb2+(0.05、0.50、0.75mg/L)单因子染毒以及Cu2++Pb2+(0.01 mg/L+0.05mg/L、0.10 mg/L+0.50 mg/L、0.25 mg/L+0.75 mg/L)联合染毒对泥鳅卵细胞DNA的损伤效应,并以SCGE技术进行检测。结果显示,Cu2+与Pb2+单因子染毒对泥鳅卵细胞DNA的损伤具有较为显著的剂量-效应与时间-效应关系(P<0.05)。Cu2++Pb2+联合染毒,在溶液暴露的0—5d表现为剂量-效应与时间-效应关系(P<0.05);Cu2++Pb2+暴露5—10d则表现出拮抗作用。研究结果显示,Cu2+、Pb2+单因子及联合染毒均造成泥鳅卵细胞DNA损伤,具有基因毒性效应。The effects of Cu^2＋ and Pb^2＋ （single factor and joint toxicity） attack on DNA damages in loach oosytes with different concentration of Cu^2＋（0.01, 0.10 and 0.25 mg/L respectively）and Pb^2＋（0.05, 0.50 and 0.75mg/L respectively）, and Cu^2＋＋Pb^2＋（0.01 mg/L＋0.05mg/L, 0.10 mg/L＋0.50 mg/L and 0.25 mg/L＋0.75 mg/L respectively）were studied by means of in door exposure and with the technique of SCGE. The results showed that the DNA damages in loach oosytes by single factor Cu^2＋ or Pb^2＋showed significant differences with dose-effect and time-effect （P〈0.05）. As for joint attack of Cu^2＋＋Pb^2＋ it showed that there were significant differences with dose-effect and time-effect under 5d exposure （P〈0.05）, but it seemed to be an antagonism within 5d to 10d exposure. The results of the study showed that both single factor or joint attack by heavy metals will cause DNA damages to loach oosytes. Meanwhile, the genetic toxicity effects appears.

关 键 词：泥鳅 卵细胞 Cu2+ PB2+ SCGE检测 DNA损伤 

分 类 号：Q255[生物学—细胞生物学]