对氨基水杨酸钠对亚慢性染锰大鼠基底前脑胆碱能神经细胞的影响  

Effects of Sodium Aminosalicylate on Basal Forebrain Choline Acetyltransferase Neurons of Rats Induced by Sub-Chronic Manganese Exposure

作　　者：蒙浩洋[1] 李少军[1] 唐方萍[1] 黄艳妮[1] 罗海兰[1] 陈静雯[1] 邓祥发[2] 姜岳明[1] 

机构地区：[1]广西医科大学公共卫生学院卫生毒理学教研室,广西530021 [2]广西医科大学基础医学院人体解剖学教研室,广西530021

出　　处：《环境与职业医学》2013年第6期461-464,共4页Journal of Environmental and Occupational Medicine

基　　金：国家自然科学基金项目(编号:81072320;30760210)

摘　　要：[目的]探讨对氨基水杨酸钠(PAS-Na)对亚慢性染锰大鼠基底前脑胆碱能神经细胞的影响。[方法]48只雄性SD大鼠被随机分为对照Ⅰ、Ⅱ组,染锰Ⅰ、Ⅱ组,PAS-Na预防(预防)组,PAS-Na治疗(治疗)组,每组8只。染锰组、预防组、治疗组大鼠每日腹腔注射氯化锰(MnCl2.4H2O 15mg/kg),每周5d,对照组腹腔注射等容量生理盐水,预防组在染锰同时每日背部皮下注射PAS-Na(200mg/kg),连续12周。13周开始,治疗组每日背部皮下注射PAS-Na(200mg/kg),同时染锰Ⅱ组、对照Ⅱ组背部皮下注射等容量生理盐水至18周。采用水迷宫实验检测大鼠学习记忆能力,免疫组化染色观察胆碱乙酰转移酶(ChAT)阳性神经元形态及数量,检测基底前脑ChAT蛋白活性。[结果]12周后,染锰组逃避潜伏期、游泳路程均比对照Ⅰ组长,预防组逃避潜伏期、游泳路程均较染锰组短(P<0.05)。染锰组基底前脑垂直支臂核(vDB)/水平支臂核(hDB)ChAT阳性细胞数较对照Ⅰ组少,预防组vDB/hDB ChAT阳性细胞数较染锰组多(P<0.05);染锰组大鼠基底前脑组织ChAT蛋白活性较对照Ⅰ组低,预防组较之回升(P<0.05)。18周后,染锰组逃避潜伏期、游泳路程都比对照组长,治疗组逃避潜伏期、游泳路程比染锰组短(P<0.05)。染锰组vDB/hDB ChAT阳性细胞数较对照组少,治疗组vDB ChAT阳性细胞数较染锰Ⅱ组多(P<0.05)。[结论]PAS-Na对亚慢性染锰引起大鼠基底前脑ChAT阳性细胞减少、ChAT蛋白活性降低和学习记忆障碍可能有拮抗作用。[ Objective ] To explore the effects of sodium aminosalicylate （PAS-Na） on basal forebrain choline acetyhransferase （CHAT） neurons of rats induced by sub-chronic Mn exposure. [ Methods ] Forty-eight male SD rats were randomly assigned into 6 groups： control group I and II, Mn-exposed group I and I], PAS-Na prevention （PAS-P） group, and PAS-Na treatment （PAS-T） group, 8 rats for each group. The Mn-exposed group, the PAS-P group, and the PAS-T group received intraperitoneal injection （ip） of MnC12 -4H20 15 mg/kg, the control group received ip of physiological saline at the same dose, and the PAS-P group received back subcutaneous injection （sc） of PAS-Na 200 mg/kg, all procedures were performed 5 days a week for 12weeks. Then, the rats in the PAS-T group received back sc of PAS-Na 200mg/kg, while the rats in the Mn-exposed group II and the control group II received back sc of physiological saline at the same volume once a day, for 6 weeks. Morris water maze was utilized to probe rats learning and memory capacity. Morphological change and ChAT positive neuron count in basal forebrain were recorded under microscope after immunohistochemistry staining, and ChAT protein activity was also detected. [ Results ] After 12 weeks, the escape latency and the swimming distance obviously increased in the Mn-exposed group I when compared with those of the control group I, but the treatment in the PAS-P group reversed the escape latency and swimming distance induced by Mn exposure （P 〈 0.05）. The ChAT positive neuron count of basal forebrain vertical limb of diagonal band （vDB）/horizontal limb of diagonal band （hDB） and ChAT protein activity in the Mn-exposed group I were lower than those in the control group I, the treatment in the PAS-P group reversed distinctly vDB/hDB ChAT positive count and ChAT protein activity induced by Mn （P 〈 0.05）. After 18weeks, the escape latency and the swimming distance obviously increased in the Mn-exposed group ]I when compared with those of the contr

关 键 词：对氨基水杨酸钠 锰 学习记忆 基底前脑 胆碱能神经细胞 

分 类 号：R114[医药卫生—卫生毒理学]