孕早期高蛋白膳食对子代大鼠PEPCK的影响  

Effects of high protein diet during early pregnancy on phosphoenolpyruvate carboxykinase of offspring rats

作　　者：董艳梅[1] 孙长颢[2] 李雅杰[3] 李省三[3] 赵剑飞[3] 冯海英[3] 刘吉成[3] 

机构地区：[1]黑龙江中医药大学博士后流动站 [2]哈尔滨医科大学 [3]齐齐哈尔医学院,黑龙江齐齐哈尔161006

出　　处：《中国妇幼保健》2013年第18期2989-2991,共3页Maternal and Child Health Care of China

基　　金：国家自然科学基金〔81130049〕;黑龙江省自然科学基金〔D200801〕;黑龙江省教育厅面上项目〔11541412〕

摘　　要：目的:研究孕早期高蛋白膳食(HPD)对后代大鼠成年肥胖及磷酸烯醇式丙酮酸羧激酶(PEPCK)表达的影响,为肥胖的早期防治提供依据。方法:孕早期大鼠分别给予HPD和标准膳食(SD),其后代分别为高蛋白质组(HP)和对照组(CON),交叉哺乳3周后给予SD,成年后高脂膳食诱导肥胖。分阶段处死大鼠取白色脂肪,荧光定量PCR法检测PEPCKmRNA的水平。结果:与SD大鼠后代相比,HPD大鼠后代的体重降低(P<0.05),PEPCK mRNA水平下降。结论:孕早期HPD可程序性降低后代PEPCK mRNA的水平,减少了成年肥胖的发生。Objective： To explore the effects of high protein diet during early pregnancy on offspring phosphoenolpyruvate car- boxykinase （PEPCK） gene, and find out the method of preventing obesity. Methods： Rats were received a high protein diet （HPD） and standard diet （SD） during early pregnancy, respectively. Their offspring were divided into high protein group （HP） and control group （ CON）, respectively. Offspring were fed with SD after weaning. At adult, rats were fed with high fat diet （HFD） to induce obesity. At dif- ferent stages, offspring were sacrificed for analyzing PEPCK gene level. Results： Body weight and PEPCK gene level of offspring of HPD during pregnancy were decreased vs offspring of SD during pregnancy （ P 〈 0.05 ） . Conclusion： HPD during early pregnancy can prevent offspring from obesity and decrease PEPCK gene level.

关 键 词：孕早期 肥胖 磷酸烯醇式丙酮酸羧激酶 子代 

分 类 号：R715.3[医药卫生—妇产科学]