致病疫霉拮抗菌荧光假单胞菌的筛选及离体防病作用  

Screening of antagonistic Pseudomonas fluorescens against Phytophthora infestans and disease control in vitro

作　　者：蒋继志[1] 梁廷银[1] 王怀远[1] 王兴哲[2] 

机构地区：[1]河北大学生命科学学院,河北保定071002 [2]河北医科大学第二医院,河北石家庄050000

出　　处：《河北农业大学学报》2013年第3期72-76,共5页Journal of Hebei Agricultural University

基　　金：河北省自然科学基金项目(C2011201003);公益性行业(农业)科研专项(201303018)

摘　　要：为了从马铃薯根际土壤中筛选显著拮抗致病疫霉且离体防病效果显著的荧光假单胞菌,本试验以稀释平板法并结合紫外光分离根际土壤中的荧光细菌,采用对峙培养及打孔法测试菌株、菌液及发酵液对致病疫霉的抑制作用,块茎切片法评价菌液的离体防病效果。结果显示:分离纯化的30株荧光细菌中有14株对致病疫霉菌丝生长有明显抑制作用,抑菌率在60%以上;其中经初步鉴定为荧光假单胞菌(Pseudomonas fluorescens)的M15菌株抑制作用最强,活体、菌液和发酵液的抑菌率分别为86.39%,88.23%和65.88%,其菌液在马铃薯块茎切片上对晚疫病的预防效果为65.06%,显微观察发现该菌株菌液使致病疫霉菌丝体畸形,并对几种常见的植物病原真菌具有明显拮杭作用。这表明M15菌株在防治马铃薯晚疫病方面具有较大的潜在应用价值。The paper was to screen antagonistic Pseudomonas fluorescens strains against Phyto- phthora infestans and controlling potato late bight in vitro. The fluorescent bacterial strains were isolated with the dilution plate method in combination with ultraviolet. Inhibition of bac- terial living strains, bacterial fluid and fermentation liquid on mycelial growth of P. infestans was tested with dual culture and punch method, respectively. Disease control effect of bacterial fluid from antagonistic strain was evaluated by P. more than 60%. In which, M15 strain identified infestans, their growth inhibition rates were as P. fluorescens, had the strongest antago- nistic effect, and the inhibition rates of its living strains, bacterial fluid and fermentation liquid suppression were 86.39%, 88.23% and 65.88%, respectively. Prevention effect of bacterial fluid on potato tuber slices against late blight was 65.06 %. Microexamination showed that bac- terial fluid of M15 strain could cause deformation of P. infestans mycelium, and was antago- nistic to other phytopathogenic fungi. These findings indicate that M15 strain has a great po- tential in control of potato late blight in future.

关 键 词：致病疫霉 拮抗菌 荧光假单胞菌 菌液 

分 类 号：S436.412.12[农业科学—农业昆虫与害虫防治]