高效液相色谱法测定土壤中二氯喹啉酸的不确定度  

Uncertainty in Determination of Quinclorac in Soil with HPLC

作　　者：黎茶根 唐明[2] 钟秋瓒 何宽信 万树青[2] 申昌优 万应发 张超群 郭伟 

机构地区：[1]江西省烟草公司,南昌市洪城路298号330025 [2]华南农业大学农药及化学生物学教育部重点实验室 [3]赣州市烟草科学研究所 [4]江西省烟叶科学研究所,南昌市安石路1号330025

出　　处：《烟草科技》2013年第6期40-44,共5页Tobacco Science & Technology

基　　金：江西省烟草专卖局(公司)科技项目"土壤中除草剂残留对烟叶生产的影响与应对措施"(201101003)

摘　　要：为提高检测结果的准确性,依据JJF 1059—1999《测量不确定度评定与表示》系统计算了高效液相色谱(HPLC)法测定土壤中二氯喹啉酸含量的不确定度。结果表明:①标准工作液配制和标准工作曲线拟合对测量不确定度影响最大;②当二氯喹啉酸含量为0.0236 mg/kg,其合成不确定度为0.00045 mg/kg,扩展不确定度为0.0009 mg/kg(置信度95%,k=2)。采用高纯度的标准品和合格的计量器具,严格控制处理步骤,可以降低HPLC法测定土壤中二氯喹啉酸的不确定度。In order to improve the accuracy of test results, the uncertainty in determination of quinclorac in soil with HPLC was systematically calculated according to JJF 1059--1999 Evaluation and expression of uncertainty in measurement. The results indicated that： 1） Preparation of standard working solution, calibration of working curve and constant volume of sample solution were the major factors influencing the uncertainty; 2） The combined uncertainty was 0.00069 mg/kg and the expanded uncertainty was 0.0014 mg/kg （level of confidence of 95%, k=2） at the concentration of quinclorac in soil of 0.0236 mg/kg. The uncertainty in determination of quinclorac in soil with HPLC could be decreased by using high purity standard substance and qualified metering devices, and strictly controlling treatment procedures.

关 键 词：不确定度 高效液相色谱(HPLC) 二氯喹啉酸 土壤 

分 类 号：S482.41[农业科学—农药学]