β3肾上腺素能受体基因及瘦素基因多态性与新疆哈萨克族儿童肥胖的关系  

Association of polymorphism of β3adrenergic receptor gene and the leptin gene with Kazak obese children in Xinjiang

作　　者：朱翠翠[1] 张季红[1] 徐佩茹[1,2] 李敏[1] 马冬梅[1] 

机构地区：[1]新疆医科大学第一附属医院儿科,乌鲁木齐830054 [2]新疆维吾尔自治区循证医学研究所

出　　处：《中华实用儿科临床杂志》2013年第11期816-819,共4页Chinese Journal of Applied Clinical Pediatrics

基　　金：新疆维吾尔自治区自然科学基金（2010211A42）

摘　　要：目的 探讨β3肾上腺素能受体基因（β3-AR基因）Trp64Arg多态性及瘦素基因启动子区 C2549A多态性与新疆哈萨克族学龄儿童肥胖的相关性。方法 选取乌鲁木齐周边地区6～12岁哈萨克族学龄肥胖儿童92例及健康对照儿童71例，应用限制性片段长度多态性方法对瘦素基因启动子区C2549A 多态性及 β3-AR基因Trp64Arg多态性进行检测。结果 1.哈萨克族学龄肥胖儿童与健康对照儿童β3-AR基因型分布的差异有统计学意义（X^2=10.472，P 〈0.05），但等位基因分布差异无统计学意义（X^2=3.541，P 〉0.05）。2.二组间瘦素基因启动子区C2549A位点基因型及等位基因分布差异均有统计学意义（X^2=13.013、4.436，P均 〈0.05）；等位基因优势比值（0.608）及95% CI0.380～0.972提示该突变对肥胖的发生可能有一定的保护作用。3.二组间β3-AR基因Trp64Arg多态性与瘦素基因启动子区C2549A多态性的共同作用表现在同一哈萨克族学龄儿童个体中的基因型分布频率的差异有统计学意义（X^2=15.978，P 〈0.05），但2组间与两基因不同等位基因的共同作用表现在同一个体中的分布差异无统计学意义（X^2=6.362，P 〉0.05）。结论 1.新疆哈萨克族学龄儿童中存在β3-AR基因Trp64Arg及瘦素基因启动子区C2549A位点多态性；2.瘦素基因启动子区C2549A位点的突变对哈萨克族学龄儿童肥胖的发生可能有一定保护作用，β3-AR基因 Trp64Arg多态性可能与哈萨克族学龄儿童肥胖相关；3.β3-AR基因Trp64Arg与瘦素基因启动子区C2549A位点多态性在同一个体的共同作用可能与哈萨克族学龄儿童肥胖有关。Objective To investigate the association between C2549A polymorphism of leptin gene and the Trp64Arg polymorphism of the β3-adrenergic receptor gene （β3-AR gene） and obesity in Kazak school-age children. Methods Totally 92 obese children and 71 healthy controls were selected from 6 to 12 years old in Kazak school-age children from the area around Urumqi. Genotype of the C2549A polymorphism of leptin gene and the Trp64Arg polymor-phism of β3-AR gene were determined by polymerase chain reaction and restriction fragment length polymorphism met-hods. Results 1. The difference in distribution of the β3-AR gene Trp64Arg genotype of obesity and healthy controls of Kazak children was statistically significant （X^2 = 10.472, P 〈 0.05）, but the difference in distribution of the alleles was not statistically significant（X^2 = 3. 541, P 〉 0.05 ）. 2. The differences in distribution of the leptin C2549A genotype and alleles of the 2 groups were all statistically significant,and the odds ratio of the alleles（0.608） and 95% CI 0. 380-0. 972 sug-gested that the mutation occurrence of obesity might have certain protective effect. 3. The difference in distribution of the genotype of β3-AR gene Trp64Arg polymorphism and leptin gene promoter area C2549A polymorphism in the same indi-vidual joint action of 2 groups was statistically significant （X^2 = 15. 978 ,P 〈 0. 05 ）, but the difference in distribution of the alleles in the same individual joint action of 2 groups was not statistically significant（X^2 = 6.362 ,P 〉 0.05 ）. Conclusions 1. There were distributions of the Trp64Arg polymorphism of β3-AR gene and the C2549A polymorphism of leptin gene in Kazak school-aged children in Xinjiang. 2. These results suggested that C2549A mutation of leptin gene might have cer-tain protective effect in Kazak children obesity, and the Trp64Arg polymorphism of β3-AR gene might be associated with Kazak children obesity. 3. These results suggested that the Trp64Arg polymorphism of β3-AR gene and the C2549A

关 键 词：瘦素基因 β2 肾上腺素能受体基因 基因多态性 肥胖 哈萨克族儿童 

分 类 号：R7[医药卫生—临床医学]