糖皮质激素受体在激素性幼兔股骨头缺血性坏死模型中的表达及意义  

Expression of glucocorticoid receptor in steroid-induced juvenile rabbit model of avascular necrosis of the femoral head and its significance

作　　者：谢祎[1] 俞松[1] 罗宇[1] 徐艳朋[1] 彭刚[1] 王健[1] 唐诗鹏[1] 

机构地区：[1]遵义医学院附属医院小儿矫形外科,563003

出　　处：《中华实用儿科临床杂志》2013年第11期835-838,共4页Chinese Journal of Applied Clinical Pediatrics

摘　　要：目的观察糖皮质激素受体（GR）在激素性幼兔股骨头缺血性坏死模型中的表达，探讨儿童股骨头缺血性坏死可能的发病原因。方法选用2月龄新西兰大白兔60只，雌雄各半，体质量1．4～1．7kg。随机分为2组，激素注射组48只，臀肌注射糖皮质激素（GC）（7．5mg／kg），每周2次，共8周。对照组12只，臀肌注射等体积9g／L盐水（0．3mL／kg），每周2次，共8周。根据是否发病将激素注射组分为激素注射发病组和激素注射未发病组，免疫组织化学法检测GR在激素注射发病组与激素注射未发病组及对照组股骨头中的表达并进行图像分析，比较各组GR表达的差异。结果注射后8周，存活31只，股骨头缺血性坏死7只，发生率22．58％（7／31只）。激素注射发病组股骨头软骨中GR阳性表达率高，为100．00％（7／7只），而激素注射未发病组、对照组阳性表达率较低，分别为20．83％（5／24只）、16．67％（1／6只），差异均有统计学意义（P均〈0．01），激素注射未发病组与对照组比较，差异无统计学意义（P〉0．05）。同时，激素注射发病组股骨头软骨中GR表达强度较高，而激素注射未发病组、对照组股骨头软骨中GR的表达强度较低，差异均有统计学意义（P均〈0．01）；激素注射未发病组与对照组比较，差异无统计学意义（P〉0．05）。激素注射发病组股骨头软骨中GR表达量较高，积分吸光度（IOD）值为7942．21±1886．94，而激素注射未发病组、对照组实验动物股骨头软骨中GR的表达量极低甚至不表达，其IOD值分别为627．56±281．85、571．48±260．22，差异均有统计学意义（P均〈0．01）；激素注射未发病组与对照组比较，差异无统计学意义（P〉0．05）。结论单纯臀肌注射GC可导致幼兔股骨头缺血坏死的发生；GC及GR可能在幼兔股骨头缺血坏死的发生、发展过程中起重�Objective To detect the expression of glucocorticoid receptor （GR） in juvenile rabbit modal of avascular necrosis of the femoral head, and explore the possible etiology of avascular necrosis of the femoral head in children. Methods Sixty New Zealand albino rabbits（50% female and 50% male） ,2 months old,body weight 1.4 - 1.7 kg,were randomly divided into 2 groups：steroid injection group（SIG） and control group（CG）. The SIG（ n = 48） ： Prednisolone acetate of 7.5 mg/kg was injected into each rabbit twice a week for 8 weeks. The CG（ n = 12） ：9 g/L sa-line of 0.3 mL/kg was injected into each rabbit twice a week for 8 weeks. The immunohistochemistry and image analysis were used to detect the expression of GR. The SIG was divided into disease group（DG） and non-disease group（NDG）. The expressions of GR were compared among DG, NDG and CG. Results In the 8^th week after the first injection in SIG,31 out of 48 rabbits survived, and 7 out of 31 rabbits （22.85 % ） developed femoral head avascular necrosis. GR expression in DG was 100.00% （7/7 cases） ,while in NDG and CG they were 20.83% （5/24 cases） and 16.67% （ 1/6 cases）. Significant difference was found between DG and NDG/CG（ all P 〈 0.01 ）, but not between NDG and CG. GR expression density in femoral cartilage in DG was higher than in NDG and CG（ all P 〈 0.01 ）. GR expression IOD in femoral cartilage was 7942.21 ± 1886.94, but in DG and NDG they were extremely low or undetectable,which were 627.56 ± 281.85 and 571.48 ± 260.22, separately, and there was significant difference （ all P 〈 0.01 ）. Conclusions Simple gluteal injection of glucocorticoid can lead to juvenile rabbits avascular necrosis of femoral head. GC and GR might play an important role in the onset and development of avascular necrosis of femoral head in juvenile rabbits.

关 键 词：股骨头缺血性坏死 动物模型 糖皮质激素 糖皮质激素受体 幼兔 

分 类 号：R681.8[医药卫生—骨科学]