尤溪金柑新品种金秋早的ISSR鉴定  

Identification of a New Cultivar Jinqiuzao of Fortunella crassifolia by ISSR Markers

作　　者：金建涛[1] 赖钟雄[1] 陈登云 卓春宣 郑运绪 

机构地区：[1]福建农林大学园艺植物生物工程研究所,福建福州350002 [2]福建省尤溪县科技局,福建尤溪365100 [3]福建省尤溪县管前镇农业服务中心,福建尤溪365116

出　　处：《园艺与种苗》2013年第4期36-40,共5页Horticulture & Seed

基　　金：福建省农业科技平台项目(2008N2001);福建省省长基金项目(szjj07);尤溪县金柑产业化关键技术研究重大科技专项(2008)

摘　　要：[目的]为金秋早金柑的品种鉴定提供科学依据。[方法]通过ISSR分子标记技术对金秋早金柑母株、嫁接子代及对照等10份尤溪金柑株系样品基因组DNA进行分析鉴定。[结果]从48条引物中共筛选出10条能扩增出清晰度高、多态性好条带的引物。10条引物共扩增出84个条带,其中多态性条带38条,多态性百分率为47.50%。金秋早母株与嫁接子代植株之间的DNA扩增图谱几乎没有差异,而母株、嫁接子代与其他尤溪金柑的DNA扩增图谱则有明显的不同,说明高接后该品种遗传性状稳定;金秋早与普通尤溪金柑在DNA水平上存在差异,为不同品种;进一步的UPGMA聚类分析结果也支持这一观点。[结论]ISSR分子标记技术可以作为尤溪金柑新品种鉴定手段之一。[Objective]The aim was to provide a scientific basis for Mentification of new cultivar Jinqiuzao of Fortunella crassifolia. [Method]DNA polynrorphism of a new cuhivar Jinqiuzao and 9 related lines （ the mother tree, grafting trees and CK trees） were determined by ISSR molecular markers in Fortunella crassifolia. Ten primers combinations were selected with the abundant polymorphism from 48 primers. [Result] The results showed that 84 DNA bands were amplified by 10 ISSR primers, in which 38 bands were polymorphic, and tire polymorphic ratio （PPB） was 47.50%. The polymorphic bands had no significant difference among the mother tree and grafting trees, but remarkable difference between Jinqiuzao and the other trees, which suggested thai the grafting trees of Jinqiuzao were stable in genetic characteristics, and Jinqiuzao was a new cultivar and different the common Youxijingan cuhivar. This result was supported by the further UPGMA cluster analysis. [Conclusion] ISSR molecular marker technology could be used as one of tire means of identification of new varieties of Fortunella crassifolia.

关 键 词：尤溪金柑 金秋早金柑 ISSR 分子鉴定 

分 类 号：S432[农业科学—植物病理学]