戒烟对烟雾暴露大鼠肺血管结缔组织生长因子及基质金属蛋白酶-2表达的影响  

Effect of smoking cessation on expression of pulmonary vascular connective tissue growth factor and matrixmetalloproteinase-2 in rats of cigarette smoke exposure

作　　者：鲁彩花[1] 胡晓芸[1] 施熠炜[1] 庞敏[1] 宋妤[1] 马爱玲[1] 

机构地区：[1]山西医科大学第一医院呼吸科,太原03000l

出　　处：《国际呼吸杂志》2013年第12期885-890,共6页International Journal of Respiration

基　　金：基金项目：山西省卫生厅科技攻关计划项目（20100201）

摘　　要：目的观察烟雾暴露后戒烟对大鼠肺血管结缔组织生长因子（CTGF）与基质金属蛋白酶-2（MMP-2）表达的影响，探讨吸烟对肺血管重塑的作用。方法雄性SD大鼠40只，随机分为对照组（C组）、烟雾暴露4周组（S1组）、烟雾暴露4周戒烟4周组（Q1组）、烟雾暴露12周组（S2组）、烟雾暴露12周戒烟4周组（Q2组），每组8只。造模成功后取肺组织，HE染色测定肺血管管壁厚度占血管外径的百分比（WT％）及管壁面积占血管总面积的百分比（WA％），免疫组化染色检测肺血管壁CTGF及MMP-2蛋白的表达，RT-PCR法测定CTGF及MMP-2mRNA的表达，并分析其相关性。结果①WT％及WA％比较：S1组[（42．48±6．17）％、（62．21±5．79）％]与s2组[（51．84±8．88）％、（74．09±10．51）％]均高于C组[（30．21±8．51）％、（48．82±10．78）％]，s2组高于s1组，差异均有统计学意义（P值均〈0．05）；Q1组[（31．86土5．74）％、（50．76±11．15）％]低于S1组，Q2组[（40．54士11．87）％、（63．39±10．95）％]低于s2组，高于Q1组及C组，差异均有统计学意义（P值均〈O．05）；Ql组与C组比较差异无统计学意义（P〉O．05）。②CTGF蛋白及mRNA表达：Sl组（O．269±0．041、1．725±0．529）与s2组（0．388±0．034、2．541±0．945）均高于c组（0．204±0．046、0．716±0．439），S2组高于S1组，差异均有统计学意义（P值均〈0．05）；Q1组（0．225±0．036、1．018±0．571）低于S1组，Q2组（0．303±o．039、1．903±0．881）低于s2组，高于ql组及C组，差异均有统计学意义（P值均〈O．05）；Q1组与c组比较差异无统计学意义（P〉O．05）。③MMP-2蛋白及mRNA表达：Sl组（O．388±0．041、1．167±0．639）与s2组（O．578±0．387、3．721±0．825）均高于C组（O．190±0．022、0．276±0．256），s2组高于sl组，差异均有统计学意义（P值均〈0．05）；Q1组（Objective To observe the effect of smoking cessation on expression of pulmonary vascular connective tissue growth factor （CTGF） and matrix metalloproteinase-2 （MMP-2） in rats which exposed cigarette smoke and to probe the roles of CTGF and MMP-2 in pulmonary vascular remodeling. Methods Forty male SD rats were randomly divided into control group （C group）, smoke exposure four weeks group （S1 group）, smoke exposure four weeks and smoke cessation four weeks group （Q1 group）,smoke exposure 12 weeks group （S2 group）, smoke exposure 12 weeks and smoke cessation four weeks group （Q2 group）, with eight rats in each group. The ratio of pulmonary vascular wall thickness/vascular external diameter （WT%） and the ratio of pulmonary vascular wall area/total pulmonary vascular area （WA%） were measured by image analysis system. The expressions of CTGF and MMP-2 protein in pulmonary vascular of each group were evaluated by immunohistochemistry. The expressions of CTGF and MMP-2 mRNA in pulmonary vascular of each group were detected by reverse transcription polymerase chain reaction. Results ①WT% and WA% in S1 group [（42.48±6.17）%,（62.21±5.79）% ] and S2 group [（51.84±8.88）%,（74.09±10.51）%] were higher than those in C group [（30. 21±8. 51） % , （48. 82 ±10. 78） %] , WT% and WA% in S2 group were higher than those in S1 group （all P 〈0.05）. WT% and WA% in Q1 group [（31.86±5.74） %, （50.76±11.15） %] were lower than those in S1 group, WT% and WA% in Q2 group [（40.54±11.87） %, （63.39±10.95）%] were lower than those in S2 group, but higher than those in Q1 group and C group （all P 〈0.05）. There was no statistical difference in WT% and WA% between Q1 group and C group （P 〉 0.05）. ② The expressions of CTGF protein and mRNA in S1 group （0. 269±0. 041,1. 725±0. 529） and S2 group （0. 388±0. 034,2. 541±0. 945） were higher than those in C group （0. 204±0. 046,0. 716±0. 439） ,the expressions of CTGF protein and mRNA in S2 g

关 键 词：烟雾暴露 肺血管 重塑 结缔组织生长因子 基质金属蛋白酶-2 

分 类 号：R285.5[医药卫生—中药学]