1株基因1型戊型肝炎病毒全基因组序列的测定及分析  

Complete genome sequence analysis of one genotype 1 HEV strain from a sporadic acute hepatitis E patient

作　　者：耿彦生[1] 马红霞[2] 赵晨燕[3] 黄维金[3] 王佑春[3] 

机构地区：[1]河北大学病原生物学系,保定071000 [2]河南省疾病预防控制中心 [3]中国食品药品检定研究院,北京100050

出　　处：《中华微生物学和免疫学杂志》2013年第6期429-433,共5页Chinese Journal of Microbiology and Immunology

基　　金：国家自然科学基金（81171549）;河北省科技计划项目（12277726）

摘　　要：目的对分离自新疆散发戊型肝炎（戊肝）患者的1株戊型肝炎病毒（HEV）W2—1全基因组序列进行测定和分析。方法应用RT—PCR分段扩增的方法获得W2．1的全长基因组序列；通过与GenBank中HEV代表株相比较，对其进行分型和基因同源性分析。结果W2．1全长7212nt，5’和3’UTR以及3个开放读码框（ORF1～3）分别为27、28、5079、1980和345nt。序列分析表明，W2—1属于基因1型1b亚型，与引起1986-1989年新疆戊肝流行的HEV序列同源性为97．2％～98．5％，与2000-2008年我国分离到的1b亚型HEV同源性为97．6％～99．2％；其ORF1～3蛋白序列中含有区别于3型和4型HEV的特异性氨基酸位点。结论W2．1属于1b亚型，新疆戊肝大流行之后1b亚型在我国人群中仍有传播，并且其基因序列稳定。Objective To sequence and analyze the full-length genome of one HEV strain, W2-1 isolated from a sporadic hepatitis E patient hospitalized in 1999 in Xinjiang, China. Methods Nested RT- PCR assays with 4 sets primers were used to amplify the entire genome. The PCR products were purified and sequenced. The full-length genome was acquired by assembling the fragmental sequences using the DNAstar 5.01 software. The genome of W2-1 was analyzed by comparing with the reference HEVs from GenBank. Results The complete genome of W2-1 is 7212 nt in length, including three open reading frames （ ORF1- 3 ） with 5079, 1980 and 345 nt respectively, 27 nt 5＇UTR and 83 nt 3＇UTR, and a 3＇ poly A tail. Phyloge- netic analysis based on full-length genome showed that W2-1 belonged to genotype 1, subtype lb. W2-1 had high homology with the HEV strains isolated in the large hepatitis E epidemic in Xinjiang in 1987-1989, sha- ring 97.2% -98.5% nucleotide identity in the full length genome. W2-1 also showed high homology with lb strains isolated in China after 2000, with 97.6% -99.2% nueleotide identity. The specific amino acid sites in ORF1-3 proteins that distinct between genotype 1 HEV and the potential zoonotic strains did not change in W2-1. Conclusion W2-1 belongs to subtype lb. The study indicates subtype lb HEV has been circulating in China in a long period after hepatitis E outbreak in Xinjiang in 1986-1989. The amino acids of ORF1-3 of subtype 1 b are conserved.

关 键 词：戊型肝炎病毒 全基因组 序列分析 基因型 

分 类 号：R51[医药卫生—内科学]