两种船用防污涂层实海挂片表面细菌群落多样性分析  

Diversity of Bacterial Community on the Surface of Marine Paint in Temperate Coastal Marine Waters

作　　者：栾鑫[1,2] 段继周[1] 陈永伟[1,2] 池振明[2] 

机构地区：[1]中国科学院海洋研究所,青岛266071 [2]中国海洋大学海洋生命学院,青岛266003

出　　处：《应用与环境生物学报》2013年第3期471-477,共7页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金项目(40976046);中国科学院重要方向性项目(KZCX2-EW-205)~~

摘　　要：应用16S rDNA基因文库技术分析了实海全浸15 d的两种船用漆A和B表面的细菌多样性.样品A表面附着细菌的OTU数为26,分别属于变形菌门和拟杆菌门,其中γ-变形菌为优势菌,占总克隆数的65.1%;样品B表面附着细菌的OTU数为27,分别属于变形菌门、拟杆菌门和浮霉菌门,其中优势菌分别为占据总克隆数38.7%的γ-变形菌和39.8%α-变形菌.B表面附着的细菌无论在种类还是数量上都多于A,这表明这种新的环境友好型防污涂料对细菌的杀伤力不如添加氧化亚铜和杀菌剂的传统船用漆.同时,在两种船用漆表面都附着了大量的解环菌属(Cycloclasticus)的细菌,这种海洋多环芳香烃(PAHs)分解菌是海水中分解萘和菲的关键菌,可以对涂层造成破坏.The bacterial community structures on the surface of two kinds of marine paint, A and B, were immersed in seawater and investigated by 16S rDNA analysis. Phylogenetic analysis of 26 OTUs showed that the bacterial phylotypes of the A sample included two phylums of bacteria, Bacteroides and Proteobacteria, with y-Proteobacteria accounting for 65.1% of the clone number. The bacterial phylotypes of sample B included three phylums bacteria, Bacteroides, Planctomycetes and Proteobacteria,with -Proteobacteria and a-Proteobacteria as the dominant components, accounting for 38.7% and 39.8%, respectively. B is a tin free self-smoothening and self-polishing antifouling paint, which does not contain any harmful active agents to environment, nevertheless, its bactericidal efficiency is lower than traditional marine paint with cuprous oxide and fungicides. In addition, we found on the surface of both marine paints Cycloclasticus, a marine polycyclic aromatic hydrocarbons （PAHs） decomposing bacteria that can biodegrade naphthalene and phenanthrene and cause damage to the coating. Fig 5, Tab 1, Ref 24

关 键 词：生物膜 船用漆 生物多样性 PCR-RFLP 生物腐蚀 

分 类 号：U668[交通运输工程—船舶及航道工程]