肠道菌群对免疫系统的影响及其群落分析方法  

Effect of Intestinal Microbes on the Immune System and the Latest Research Methods

作　　者：陈丹丹[1,2] 顾胜华[2] 张金娜[1] 宝福凯[1] 汤岳琴[2] 吴晓磊[1,2] 

机构地区：[1]昆明医学院微生物学与免疫学教研室,昆明650031 [2]北京大学工学院,北京100871

出　　处：《应用与环境生物学报》2013年第3期542-546,共5页Chinese Journal of Applied and Environmental Biology

摘　　要：肠道复杂的微生物生态系统与机体免疫系统之间关系极为密切.研究揭示肠道微生物不但通过防止病原性微生物的入侵来调节肠道粘膜分泌SIgA(Secretory immunoglobulin A)作用于肠道免疫系统,而且还影响肠道外免疫系统,在机体自身免疫疾病的形成中也起着重要作用.关于肠道微生物群落结构研究,发展了基于16S rRNA基因序列的分子生物技术,比如近年来的焦磷酸测序、基因芯片及宏基因组测序等方法,各有其优势.未来通过生态学、微生物学和先进计算方法等跨学科结合分析所获得的肠道微生物群落的大量实验数据,将有望为人类免疫系统疾病的预防和治疗提供新策略.The complex intestinal microbial ecology is closely related with the immune system of organisms. Gut flora not only regulates the secretion of IgA to influence intestinal mucosal immunity by preventing invasion of pathogenic microorganisms, but also affects extra-intestinal immune system and plays an important role in the development of autoimmune diseases. The gut flora is usually studied with molecular biological techniques based on 16S rRNA, such as pyrosequencing, gene chip and metagenomic sequencing methods, each with its own advantages. Future experimental data of gut flora acquired with cross- disciplinary approaches of ecology, microbiology and advanced computational methods are expected to provide prevention and treatment for immune diseases. Ref49

关 键 词：肠道微生物 免疫系统 自身免疫病 16S RDNA 宏基因组学 

分 类 号：R37[医药卫生—病原生物学]