葡萄籽原花青素对肾血管性高血压大鼠血管重塑保护作用机制的研究  

Protective mechanism of grape seed procyanidin on vascular remodeling in renovascular hypertensive rats

作　　者：张妍[1] 孙柳青[1] 吴秀香[1] 唐剑涛[2] 靳俊峰[1] 冯涛[3] 

机构地区：[1]遵义医学院珠海校区病理学与病理生理学教研室,广东珠海519041 [2]遵义医学院珠海校区计算机教研室,广东珠海519041 [3]枣庄科技职业学院医学技术系,山东滕州277500

出　　处：《中国现代医学杂志》2013年第8期28-32,共5页China Journal of Modern Medicine

基　　金：贵州省科技厅资助项目(No:黔科合J字[2008]2317);贵州省高层次人才科研条件特助经费资助项目(No:2008TZJF58)

摘　　要：目的探讨葡萄籽原花青素(GSP)对肾血管性高血压大鼠血管重塑保护作用机制。方法采用两肾一夹(2K1C)法,复制肾血管性高血压(RH)大鼠模型,并设立假手术组(control,n=7)。术后2周,选取鼠尾动脉收缩压(SBP)升至130 mmHg以上的大鼠28只为RH大鼠,并随机分为4组(n=7):高血压模型组(RHmodel)、GSP低剂量治疗组[low GSP,50 mg/(kg.d)]、GSP高剂量治疗组[high GSP,200 mg/(kg.d)]和卡托普利阳性对照治疗组([captopril,30 mg/(kg.d)]。治疗6周后,分别测定各组大鼠尾动脉SBP、胸主动脉中膜厚度(MT)及管腔内径(LD);ELISA法测定腹主动脉中超氧化物歧化酶(SOD)活性、总抗氧化能力(T-AOC)、丙二醛(MDA)及一氧化氮(NO)含量;Western blotting法检测腹主动脉中白介素-10(IL-10)的蛋白表达。结果治疗6周后,与control组比较,RH model组大鼠尾动脉SBP、MT及MDA含量明显升高,而腹主动脉中SOD活性、T-AOC、NO含量、LD和IL-10的蛋白表达明显降低。与RH model组比较,GSP能显著降低大鼠尾动脉SBP、MT及MDA含量,升高腹主动脉中SOD活性、T-AOC、LD和IL-10的蛋白表达。NO含量虽然增多,但无统计学意义。结论 GSP能显著降低RH大鼠的尾动脉SBP,同时可以通过增强大鼠抗氧化能力,增加腹主动脉中NO含量及抑炎因子IL-10的蛋白表达来发挥保护血管重塑的作用。[Objective] To explore the protective mechanism of grape seed procyanidin on vascular re- modeling in renovascular hypertensive （RH） rats. [Methods] The RH model was established by two-kidney one clip （2K1C） method. 7 rats with sham operation were served as controls. Two weeks after operation, 28 rats were selected according to the increased tail systolic pressure （SBP） above 130 mmHg and randomly di- vided into 4 groups （n =7）： renovascular hypertensive model group （RH model）, low GSP-treated group （low GSP, mg/kg＇d）, high GSP-treated group （high GSP, 200 mg/kg·d） and Captopril-treated group （Captopril, 30 mg/（kg·d）. The SBP, medial thickness （MT） and luminal diameter （LD） in thoracic aortic wall were determined 6 weeks after treatment. ELISA method were used to detect the activity of superoxide dismutase （SOD）, total antioxidant capacity （T-AOC）, the content of malonaldehyde （MDA） and nitrous oxide （NO） level in abdominal aortic tissue. Western blotting was performed to detect the expressions of interleukin-10 （IL-10） protein in aortic tissue. [Results] 6 weeks after treatment, compared with control group, SBP, MT, the content of MDA levels in aortic tissue in RH model group were increased significantly, but the activity of SOD, T-AOC, NO levels and LD were reduced as well as the expressions of IL-10 protein. Compared with RH model group, treatment with GSP reduced SBP, MT and the content of MDA, increased the activity of SOD, T-AOC and LD, as well as the expressions of IL-10 protein. NO level were increased but had no significant. [Conclu- sion] GSP can decrease SBP significantly, and plays a protective role on vascular remolding by increasing antioxidative ability, the content of NO and the expression of anti-inflammatory cytokine IL-10 in aortic ves- sel in the RH rats.

关 键 词：葡萄籽原花青素 血管重塑 氧化应激 一氧化氮 白介素-10 

分 类 号：R544.1[医药卫生—心血管疾病] R282.71[医药卫生—内科学]