EV71通过2A蛋白酶切割Nup62而抑制核转运  

The 2A Protease of Enterovirus 71 Cleaves Nup62 to Inhibit Nuclear Transport

作　　者：张亚洲[1,2] 甘星[2] 宋娟[2] 孙鹏[2] 宋芹芹[2] 李公启[2] 盛琳君[2] 王宝栋[2] 卢明枝[2] 李灵敏[1] 韩俊[2] 

机构地区：[1]山西医科大学病理学教研室,太原030001 [2]中国疾病预防控制中心病毒病预防控制所传染病预防控制国家重点实验室,北京102206

出　　处：《病毒学报》2013年第4期421-425,共5页Chinese Journal of Virology

基　　金：传染病重大专项(2011ZX10004-001);重点实验室发展基金(2011SKLID104)资助

摘　　要：为了解EV71病毒对细胞核转运机制的影响,本研究构建了具有核定位信号的绿色荧光蛋白表达载体(pG-FP-NLS)。将此表达载体转染细胞后,使用EV71病毒感染转染细胞,结果发现EV71病毒可以有效阻止绿色荧光蛋白的核转移。将EV71病毒的2A蛋白酶真核表达载体与pGFP-NLS共转染RD细胞,可以发现2A蛋白酶可阻止具有核定位信号的绿色荧光蛋白的核转移而使绿色荧光蛋白表达于细胞浆。为了进一步研究病毒阻止核转移的机制,病毒感染细胞或通过转染2A蛋白酶真核表达载体进行Nup62的Western blotting检测,结果显示病毒以及2A蛋白酶均可引起Nup62表达下降。证明EV71可通过2A蛋白酶切割Nup62从而抑制核转运。To study the impact of the enterovirus 71（EV71） on the nuclear transport mechanism, The pGFP- NLS vector with nuclear location signal（NLS） was constructed, RD cells transfected by the pGFP-NLS vector were inoculated with the EV71 or cotransfected by EV71-2A vector. The results showed that GFP protein with NLS was expressed in the cytoplasm due to the inhibition of nuclear transport. In order to fur- ther study the mechanism of the EV71 to prevent nuclear transport,Nup62 was detected by Western blot- ting after RD ceils were infected with EV71 or transfected by EV71-2A vector. The results showed that decreased expression of Nup62 could be detected after infection with EV71 and transfection by EV71-2A vector. This study demonstrates that the cleavage of Nup62 by EV71 2A protease may be the mechanism of nuclear transport inhibition.

关 键 词：肠道病毒71型 Nup62 核转运 2A 

分 类 号：R373.2[医药卫生—病原生物学]