新型雌激素受体ER-α36在新生与成年大鼠海马和皮层中表达的比较  

Diverse expression of ER-α36,a novel variant of ER-α,in hippocampus and cortex of neonatal and adult rats

作　　者：刘洋[1] 方晨[1] 邹萍[2] 马依妮[1] 韩丹女[1] 嵇志红[3] 梁小峰[1] 关心[1] 黄亮[1] 冯田[1] 王一婷[4] 刘晶[5] 邹伟[1,6] 

机构地区：[1]辽宁师范大学生命科学学院,大连116081 [2]大连大学师范学院,大连116622 [3]大连大学医学院,大连116622 [4]复旦大学生命科学学院,上海200433 [5]大连医科大学附属第一医院中英再生医学中心,大连116011 [6]辽宁师范大学发展和教育心理学研究中心,大连116029

出　　处：《生理学报》2013年第3期263-268,共6页Acta Physiologica Sinica

基　　金：supported by the National Natural Science Foundation of China(No.30970353);the Key Laboratory Project of Department of Education of Liaoning Province;China(No.LS2010095);the College Students' Innovative Project of Educational Department;Liaoning Province;China(No.CSXL201210165007)

摘　　要：ER-α36是一种新型雌激素受体亚型,广泛分布于乳腺、子宫、消化道、呼吸道等组织和细胞中。本文旨在研究ER-α36在中枢神经系统中的表达和分布情况。采用免疫细胞/组织化学方法和蛋白质免疫印迹杂交比较ER-α36在新生(1日龄)与成年(12周龄)Sprague-Dawley(SD)大鼠海马及皮层区的表达和分布情况。研究显示,ER-α36在成年大鼠海马及皮层区均有表达,主要分布于锥体神经元。ER-α36在新生大鼠海马和皮层神经元中均定位于细胞膜。新生大鼠皮层神经元ER-α36蛋白表达高于海马,而成年大鼠皮层神经元ER-α36蛋白表达低于海马。与新生大鼠相比较,成年大鼠海马和皮层神经元ER-α36表达均显著提高。以上结果提示,ER-α36参与介导神经元膜雌激素信号通路的调节,且在大鼠出生后海马及皮层发育中发挥着重要作用。因此,ER-α36可能为学习记忆、神经系统退行性疾病等的防治提供潜在的药物靶点。ER-α36, a novel variant of ER-α, is expressed in breast, uterus, digestive tract, respiratory tract etc. The aim of the present study was to investigate the distribution and expression of ER-α36 in the central nervous system （CNS）. Here, we comparatively analyzed the expression pattern of ER-α36 in the hippocampus and cortex of neonatal （1-day-old） and adult （12-week-old） Sprague-Dawley （SD） rats by using immunohistochemistry/immunocytochemistry analysis and Western blot. The results showed that ER-α36 was expressed both in hippocampus and cortex of adult rats, but mainly distributed in pyramidal neurons. ER-α36 was mainly located on the cytomembrane of hippocampal and cortical neurons from neonatal rats. Compared with the cortical neurons, the hippocampal neurons showed lower ER-α36 protein expression in the neonatal rats, but exhibited higher level of ER-α36 in the adult rats. Furthermore, the adult rats showed higher levels of ER-α36 expression in both hippocampus and cortex compared with the neonatal rats. These results suggest that ER-α36 might be involved in the regulation of membrane-initiated estrogen signaling throughout the postnatal development of diverse brain regions, and thus will be a potential target for the treatment of degenerative diseases in nervous system.

关 键 词：ER-α36 中枢神经系统 出生后脑发育 原代培养神经元 

分 类 号：R96[医药卫生—药理学]