影响猪免疫性状的单倍型关联分析  

Haplotype Association Study for Immune Traits in Swine

作　　者：罗维真[1,2] 陈少康 张龙超[1] 程笃学 颜华[1] 李勇[1] 王立刚[1] 宋欣[4] 马小军[1] 刘欣[1] 李稳[1] 梁晶[1] 赵克斌[1] 王立贤[1] 

机构地区：[1]中国农业科学院北京畜牧兽医研究所,北京100193 [2]三元种业畜牧研究院,北京100192 [3]北京市畜牧兽医总站,北京100107 [4]四川农业大学动物医学院,雅安625014

出　　处：《畜牧兽医学报》2013年第6期843-852,共10页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家十二五科技支撑计划项目(2011BAD28B01);现代农业产业技术体系;中国农业科学院基本科研业务增项(2012ZL069)

摘　　要：旨在缩小课题组前期全基因组关联分析(Genome-wide Association Study,GWAS)结果区间范围,为下一步大规模群体验证提供有效候选基因。应用GWAS检验出的显著SNP标记构建单倍型,在大白猪×民猪F2设计资源群体内开展其与4个血常规指标(红细胞压积(Haematocrit,HCT)、血红蛋白(Hemoglobin,HGB)、平均红细胞血红蛋白量(Mean Corpuscular Hemoglobin,MCH)、平均红细胞体积(Mean Corpuscular Volume,MCV))和3个T细胞亚群指标(CD4+CD8-CD3+、CD4-CD8+CD3+和CD4+CD8-CD3+/CD4-CD8+CD3+)合计7个免疫性状指标的关联分析,寻找显著单倍型区段中的候选基因。分析结果表明:7号染色体上的3个单倍型区段均与T细胞亚群指标关联极显著,单倍型区段3(block7-3)与HGB及HCT关联极显著;8号染色体上3个单倍型区段均与MCH和MCV关联极显著,并由此定位5个候选基因:影响T细胞亚群指标的ABCF1和PPP1R10基因,影响HGB及HCT的GRM4基因,影响MCH及MCV的ALB和AFP基因。结合上述结果及基因功能,定位的5个基因可作为候选基因开展下一步研究。The aim of the present study was to narrow down the range of previous genome wide association study （GWAS） , and to provide valid candidate genes for the large-scale group valida- tion. Seven immune traits including four blood parameters （hematocrit （HCT）, hemoglobin （HGB）, mean corpuscular hemoglobin （MCH）, mean cell volume （MCV）） and three T lympho- cyte subpopulation parameters（CD4 ＋ CD8-CD3＋, CD4-CD8 ＋ CD3 ＋ and CD4 ＋ CD8-CD3 ＋/ CD4-CD8-t-CD3＋） were measured in a porcine intercross population （Large White × Min zhu）. Six haplotype blocks were built using the significant SNPs tested in the previous GWAS. Associ-ation studies between the haplotype blocks and the immune traits were performed. The result showed that the three blocks on SSC7 were significantly associated with the three T lymphocyte subpopulation parameters, the blockT-3 on SSC7 was significantly associated with HGB and HCT, and the three blocks on SSC8 were significantly associated with MCH and MCV. The six blocks contained five genes ABCF1, PPPIRIO, GRM4, ALB and AFP. Combination of these results, the five genes could be used as potential candidate genes for the study on immune traits.

关 键 词：免疫性状 单倍型 关联分析 猪 

分 类 号：S828[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]