山羊子宫内膜细胞与性腺激素对uNK细胞分泌活性的调节作用  

Regulatory Role of Goat Endometrial Cells and Sex Gland Hormones in uNK Cells Secretion

作　　者：廖庆红[1] 丁培阳[1] 赵丹丹[1] 王爱华[1] 齐雪峰[1] 

机构地区：[1]西北农林科技大学动物医学院,杨凌712100

出　　处：《畜牧兽医学报》2013年第6期866-870,共5页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30901089);国家博士点基金资助项目(20090204120011);中央高校基本科研业务费专项重点项目(ZD2013008)

摘　　要：为探讨山羊子宫内膜细胞和性腺激素对子宫自然杀伤(uNK)细胞分泌血管内皮生长因子(VEGF)和γ-干扰素(IFN-γ)含量的影响。本研究以永生化山羊子宫内膜基质细胞(ESC)和上皮细胞(EEC)为体外研究模型,通过ELISA法检测子宫内膜细胞和性腺激素(E2和P4)对uNK细胞分泌VEGF和IFN-γ含量的影响。结果显示,在uNK细胞与EEC共培养时,雌激素E2单独或与P4共同作用可显著抑制uNK细胞中IFN-γ和VEGF分泌水平(P<0.05),而P4单独作用不显著;而当uNK细胞与ESC共培养时,孕酮P4单独或与E2共同作用可显著增强IFN-γ在uNK细胞中的分泌水平(P<0.05),而E2单独或与P4共同作用可显著抑制VEGF分泌水平(P<0.05)。研究结果表明性腺激素对与EEC或ESC共培养的uNK细胞分泌水平具有不同程度的调节作用。To explore the effects of goat endometrial ceils and sex hormones on secretion content of VEGF and IFN-y in uNK cells. Using immortalized goat endometrial stromal cells （ESC） and epithelial cells （EEC） as an in vitro research model, the effects of the endometrial cells and sex hormones （E2, P4 ） on secretion content of VEGF and IFN-y, in uNK cells was detected by ELISA assay. When uNK cells co-cultured with EEC, estrogen alone or together with P4 significantly in- hibited the secretion levels of IFN-7 and VEGF in uNK cells （P〈0.05）, while the P4 alone had no significant effect. When uNK cells co-cultured with ESC, P4 alone or together with E2 can sig- nificantly enhance the secretion of IFN-y, in uNK cells （P〈0.05）, whereas E2 alone or together with P4 significantly inhibited the secretion level of VEGF （P〈0.05）. These results indicated that the sex hormones play a role in regulatoring secretion of uNK cells co-cultured with EEC or ESC.

关 键 词：山羊 子宫内膜上皮细胞 性腺激素 UNK细胞 分泌活性 

分 类 号：S827[农业科学—畜牧学] Q813.1[农业科学—畜牧兽医]