黑素皮质素受体1(MC1R)基因与VC-獭兔毛色关系的研究  

Study on the Relationship between the Melanocortin 1 Receptor Gene(MC1R) and the Coat Color in Rex-rabbits

作　　者：高荣琨[1] 陈伟[2] 曲海娥[2] 李建平[3] 张巧灵[2] 

机构地区：[1]山西农业大学动物科技学院,太谷030801 [2]吉林大学畜牧兽医学院,长春130062 [3]吉林农业大学动物科学技术学院,长春130118

出　　处：《畜牧兽医学报》2013年第6期888-893,共6页ACTA VETERINARIA ET ZOOTECHNICA SINICA

基　　金：国家自然科学基金(30800807;31072097);中国博士后科学基金(20100471261)

摘　　要：为研究黑素皮质素受体(Melanocortin1receptor,MC1R)基因对獭兔毛色的影响,本试验采用RT-PCR技术克隆了MC1R基因的cDNA片段,发现MC1R基因长951bp,编码317个氨基酸的前体蛋白,蛋白质PI为9.26,分子量36.28ku,具有7个跨膜结构域,与其他动物MC1R蛋白性质相符。其氨基酸序列经同源性比对结果显示:其与牦牛,马、绵羊、骆驼、牛等哺乳动物的同源性均高于93%,有14个突变频率较高的位点,其中第42、82、176、18、185位点为VC-獭兔特异性突变位点,表明该序列在哺乳动物中较为保守。但这些突变位点均发生极性、亲水疏水性等变化,这些突变可为进一步探测不同群种间的MC1R基因及该基因与獭兔毛色的关系提供理论依据。The study aimed to study the relationship between the melanocortin 1 receptor gene （MC1R） and the coat color in rex-rabbits. According to the sequence of MC1R in GenBank,a pair of specific primer was designed to amplify the cDNA sequence of MC1R of rex-rabbits using RT- PCR technique. The results showed that the 951 bp cDNA sequence encoded 317AA, isoelectric point of MC1R protein was 9.26. Compared with the published sequence in GenBank, there were 7 transmembrane domains. Homology of nucleotide sequence and deduced amino acid was higher than 95% and 93% with cattle, horse, yak, sheep, camel, and so on, which was confirmed by the analysis of phylogenetic tree. The mutation frequency of 14 loci were higher, and 5 amino acid substitutions were detected in rex-rabbits, which lies on the transmembrane domains, which showed that the sequence of MC1R was highly conservative in mammalian. These mutation loci provide the theotrtical basis for studying the relationship between MC1R and coat color in rex- rabbits.

关 键 词：VC-獭兔 MC1R RT-PCR 毛色 

分 类 号：S829.1[农业科学—畜牧学] S813.3[农业科学—畜牧兽医]