鞘脂激活蛋白原(PSAP)在多囊卵巢综合征模型大鼠卵巢中的表达及意义  

Expression and Significance of Prosaposin (PSAP) in A Rat Model of Polycystic Ovary Syndrome

作　　者：方媛媛[1] 李一宁[1] 叶莹心[1] 王秀霞[1] 

机构地区：[1]中国医科大学附属盛京医院妇产科,沈阳110004

出　　处：《生殖与避孕》2013年第6期372-377,共6页Reproduction and Contraception

基　　金：辽宁省教育厅高等学校科研项目;项目号:LT2010106

摘　　要：目的:探讨鞘脂激活蛋白原(PSAP)在多囊卵巢中的表达。方法:用免疫组织化学、RT-PCR和Western blotting方法检测来曲唑(LE)诱导的多囊卵巢综合征(PCOS)大鼠模型卵巢组织的PSAP表达。结果:LE诱导的PCOS大鼠模型内分泌紊乱,代谢特点和形态特征均与人的PCOS相似。在PCOS大鼠卵巢中,PSAPmRNA和蛋白表达均增高。结论:PSAP在LE诱导的PCOS大鼠模型卵巢组织中过表达,并在PCOS发生、发展过程中起到重要的作用。Objective： To investigate the prosaposin （PSAP） expression in polycystic ovaries. Methods： The expression of PSAP was examined using immunohistochemistry, quantitative RT-PCR and Western blotting in a rat model with letrozole-induced polycystic ovary syndrome （PCOS）. Results： Letrozole-induced PCOS rat models were similar to those in human PCOS in endocrine disturbances, metabolic characteristics and morphology features. And in ovaries of PCOS rat models both mRNA and protein levels of PSAP were increased significantly. Conclusion： PSAP was overexpressed in the letrozole-induced PCOS rat model and might play important roles in occurance and development of PCOS.

关 键 词：多囊卵巢综合征(PCOS) 雄激素受体(AR) 鞘脂激活蛋白原(PSAP) 大鼠 模型 

分 类 号：R711.75[医药卫生—妇产科学]