RNAi介导的pirin低表达降低了K562细胞的红系分化能力  

RNA interference-mediated pirin low expression reduces the erythroid differentiation of K562 cells

作　　者：毛群铨[1] 王婕[1] 曹玲玲[1] 薛建有[1] 戚武林 朱晓芳[1] 张世馥[1] 

机构地区：[1]浙江理工大学生命科学学院,浙江杭州310018

出　　处：《基础医学与临床》2013年第7期888-891,共4页Basic and Clinical Medicine

基　　金：浙江省生物医学开放基金(SWYX0902)

摘　　要：目的研究pirin低表达对人红白血病细胞系K562红系分化和细胞活力的影响。方法通过短发卡RNA(shRNA)干扰技术,构建pirin低表达细胞稳定株,用Western blot方法对干扰效果进行检测,并用联苯胺染色和MTT法分别检测干扰pirin后对K562细胞的红系分化和增殖的影响。结果转染了pirin-shRNA的细胞其pirin的表达量明显低于对照组(随机干扰组),pirin低表达与对照组相比对细胞增殖无影响,但在诱导剂诱导分化过程中,血红蛋白的合成出现了显著性的降低(P<0.05)。结论 Pirin低表达不影响K562细胞的增殖能力,但能降低其红系分化能力。Objective This article is aimed at researching the influence of pirin knocking down on the differentia- tion and proliferation of K562 cells. Methods Techniques of shRNA interference was applied to generate stable cell strain of pirin low expression. Western blot was used to analyze the interference efficiency of pirin-shRNA. MTF and benzidine staining were applied to detect the cell viability and hemoglobin synthesis of the stable cell line in the presence of inducers. Results Western blot showed that the pirin knockdown stable cell strain had a lower expression of pirin as compared to the control group, MTT results showed that there was no difference between the pirin knockdown cell strain and the control group, but the hemoglobin synthesis had been reduced as compared to the control group. Conclusions Pirin knocking down does not affect the cell viability of K562 cells, but inhibit the erythroid differentiation of K562 cells.

关 键 词：K562细胞 慢病毒 pirin 红系分化 白血病 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]