SPE-LC-MS/MS法测定尿液与血液中海洛因3种代谢物  

Determination of morphine3-glucuronide,morphine and O6-monoacetylmorphine in urine and plasma by LC-MS/MS with molid phase extraction

作　　者：于晓巍[1] 王玫[2] 谭晓辉[1] 张大明[2] 

机构地区：[1]山西医科大学法医学院,山西太原030001 [2]北京市公安局法医鉴定中心,北京100192

出　　处：《中国法医学杂志》2013年第3期233-236,共4页Chinese Journal of Forensic Medicine

摘　　要：目的采用SPE-LC-MS/MS方法,同时检测尿液与血液中海洛因主要代谢物3-β-D-葡萄糖醛酸吗啡(M3G)、吗啡和O6-单乙酰吗啡(O6)。方法采用BAKERBONDTMspe Octadecyl(C18)进行提取,应用LC-MS/MS方法检测并通过MRM及内标法进行量化。结果尿液中M3G、吗啡、O6-单乙酰吗啡的最低检测限(LOD)分别为1.24pg、6.71pg、0.47pg;回收率依次为82.25±12.25%、93.75±13.25%、88.70±11.90%。血液中M3G、吗啡、O6-单乙酰吗啡的最低检测限分别为1.50pg、8.21pg、0.52pg。回收率依次为89.85±21.15%、73.70±17.90%、90.10±3.90%。结论本文所建方法同时适用于尿液与血液中海洛因主要代谢物M3G、吗啡、O6-单乙酰吗啡的提取、净化、分析。Objective To use the method which can accurately measure the metabolites of heroin （including morphine, morphine3-glucuronide and O6-Monoacetylmorphine） in urine and plasma by LC-MS/ MS with solid-phase extraction. Methods the samples were extracted by BAKERBONDTM spe Oetadecyl （ C8 ） Disposable Extraction Columns 3cc 500mg and then detected by LC-MS-MS, quantification of compounds was achieved using multiple reactionmonitoring （ MRM ） incorporating the use of deuterated 06- Monoacetylmorphine as internal standards. Results Limits of Detection （LOD） were 1.24pg for M3G, 6.71pg for morphine and 0. 47pg for O6-Monoacetylmorphin in urine and 1.5pg for M3G, 8.21pg for morphine and 0.52pg for O6-Monoacetylmorphin in plasma. In urine the recoveries for M3G, morphine, 06- Monoaeetylmorphine were 82.25 ± 12. 25% ,93.75 ± 13.25% ,88.70 ± 11.90% respectively, and in plasma were 89. 85 ±21.15%, 73.70 ± 17.90%, 90. 10± 3.90%. Conclusion The method is suitable for extraction, purification and quantitative analysis of morphine, morphine3-glucuronide and 06- Monoacetylmorphine both in human urine and plasma.

关 键 词：法医毒物分析 3-β-D-葡萄糖醛酸吗啡 吗啡 O6-单乙酰吗啡 SPE-LC-MS MS 

分 类 号：D919[医药卫生—法医学]