CBA法研究HLA全相合与单倍体相合移植患者血清细胞因子表达的差异性  

Different Changes of Serum Cytokines Following HLA-identical and HLA Haploidentical Non-myeloablative Allogeneic Hematopoietic Stem Cell Transplantation

作　　者：赵月莹[1] 康严[1] 孙琪云[1] 董征[1] 刘铁强[1] 郭梅[1] 刘广贤[1] 艾辉胜[1] 

机构地区：[1]军事医学科学院附属医院血液科,北京100071

出　　处：《中国实验血液学杂志》2013年第3期721-727,共7页Journal of Experimental Hematology

摘　　要：本研究旨在探讨非清髓异基因造血干细胞HLA全相合移植(HLA-identical nonmyeloablative allogeneichematopoietic stem cell transplantation,iNAHSCT)和单倍体相合移植(HLA-haploidentical nonmyeloablative allogeneichematopoietic stem cell transplantation,hiNAHSCT)患者血清细胞因子表达的差异性,用流式细胞微珠阵列法(flow cy-tometric bead array,CBA)检测20例白血病患者移植前后不同时间点IL-2、IL-4、IL-6、IL-10、TNF-ɑ、γ-IFN、IL-17浓度的变化。结果表明,IL-2分别在iNAHSCT及hiNAHST后1周、2周起表达上调,两类移植各时间点比较无差异(P>0.05);γ-IFN都在移植后4周起上调,两类移植后的浓度无差异(P>0.05);IL-4在iNAHSCT及hiNAHSCT中分别于移植后2周、移植后1周起增高,iNAHSCT的IL-4浓度高于hiNAHSCT(P<0.01);IL-6在iNAHSCT及hi-NAHSCT中分别在移植后1周、移植后2周起上调,移植后4周为峰值,且hiNAHSCT的IL-6浓度高于iNAHSCT(P<0.02);IL-10在iNAHSCT、hiNAHSCT中分别于从移植后1、2周起增高,iNAHSCT中IL-10的表达高于hiNAH-SCT(P<0.05)。TNF-ɑ在hiNAHSCT中从移植后1周、在iNAHSCT中从移植后2周起上调;hiNAHSCT中TNF-ɑ增高的幅度高于iNAHSCT(P<0.01)。IL-17分别于hiNAHSCT及iNAHSCT后移植后1周、移植后4周时增高,hi-NAHSCT IL-17增高的幅度高于iNAHSCT的(P<0.05)。结论:两类移植后所检测到的细胞因子水平均明显增高,移植后4周为峰值。IL-6、TNF-ɑ、IL-17在hiNAHSCT中增高明显,而IL-4,IL-10在iNAHSCT中增高显著。This study was aimed to investigate the expression difference of serum cytokines in 20 patients receiving HLA-identical nonmyeloablative allogeneic hematopoietic stem cell transplantation（iNAHSCT） and HLA-haploidentical nonmyeloablative allogeneic hematopoietic stem cell transplantation（hiNAHSCT）. IL-2, IL-4, IL-6, IL-10, TNF-a, ^- IFN and IL-17 were detected by flow cytometric bead array before and on week 1,2, 4 after transplantation respectively. The results showed that the IL-2 level was found to be up-regulated at week 1 and 2 after transplantation in iNAHSCT group and in hiNAHSCT group respectively, but there was no difference between these two groups （ P 〉 0.05 ）. The ^- IFN levels was up-regulated at week 4 after transplantation in above-mentioned two groups, but no difference was found between these two groups. The IL-4 level increased at week 2 and 1 after transplantation in iNAHSCT and hiNAHCT groups respectively, but the IL-4 level in iNAHSCT group was higher than that in hiNAHSCT group. The IL-6 level rose at week 1 and 2 after transplant in above mentioned groups respectively, and reached to peak level at week 4 after trans- plantation, but 1L-6 level in hiNAHSCT was higher than that in iNAHSCT group （P 〈0.02）. The IL-10 level was up- regulated at week 1 and 2 in iNAHSCT and hiNAHSCT groups respectively, but the IL-10 level in iNAHSC was higher than that in hiNAHSCT group. The TNF-ct level was up-regulated at week 1 in hiNAHSCT group, but at week 2 in iN- AHSCT group after transplantation. The TNF-a level in hiNHASCT group was higher than that in iNAHSCT group （ P 〈 0.01 ）. The IL-17 level was up-regulated at week 1 and week 4 after transplantation in hiNAHSCT and iNAHSCT groupsrespectively, the IL-17 level in hiNAHSCT group was high as compared with that in iNAHSCT group. It is concluded that the serum cytokine levels are obviously up-regulated in iNAHSCT and hiNHASCT groups, and reach to peak level at week 4 after transplantation. The IL-6, TNF-a and IL-17 level up-regula

关 键 词：非清髓异基因造血干细胞移植 HLA全相合异基因造血干细胞移植 单倍体相合异基因造血干细胞移植 细胞因子 

分 类 号：R457.7[医药卫生—治疗学]