WHBE免与日本大耳白兔肠道淋巴细胞免疫活性的比较研究  

Comparative Study on Immunological Functions of Intestina Parva Between WHBE Rabbit and Japanese White Rabbit

作　　者：胡慧颖[1] 王志远[1] 柯贤福[1] 余陈欢[1] 屠珏[2] 张利棕[2] 应华忠[1] 

机构地区：[1]浙江省医学科学院,杭州310013 [2]浙江中医药大学,杭州310005

出　　处：《实验动物与比较医学》2013年第3期215-217,共3页Laboratory Animal and Comparative Medicine

基　　金：浙江省卫生厅项目（2007QN013）;浙江省卫生高层次创新人才培养工程项目

摘　　要：目的比较白毛黑眼（WHBE）与日本大耳白（JW）兔肠道淋巴细胞免疫活性的差异。方法分别选取离乳和2月龄雄性WHBE兔、Jw兔各8只，采用percoll分离液和Ficoll分离液，分别测定其小肠上皮层淋巴细胞；MTT法检测刀豆蛋白A（ConA）、脂多糖（LPS）对兔小肠上皮层淋巴细胞增殖的影响。结果成年WHBE兔小肠淋巴细胞受ConA、LPS刺激的增殖程度比成年Jw兔的明显升高，具有显著性差异。结论WHBE兔的肠道淋巴细胞较Jw兔更易受丝裂原的刺激作用而诱导激活，这可能是其肠易激的原因之一。Objective To investigate differences on immunological function of small bowel between white hair black eye （WHBE） rabbit and Japanese white （JW） rabbit. Methods The weanling and 2 months old male WHBE rabbits and JW rabbits were used with 8 in each group. Two kinds of cell separation mediums￡ficoll and percoll ,were adopted for the isolation and purification. MTT test was applied to detect the lymphocyte proliferation induced by ConA and LPS. Results The levels of lymphocyte proliferation in small bowel of grown WHBE rabbits were much higher than those of grown JW rabbit. Conclusions Immunological function of lymphocytes in small bowel of grown WHBE Rabbit was much weaker than that of Japanese Rabbits. It may warrant further evaluation as a possible animal for the research of irritable bowel syndrome.

关 键 词：白毛黑眼兔 日本大耳白兔 淋巴细胞 免疫功能 

分 类 号：Q95-33[生物学—动物学]