哺乳动物乳腺上皮细胞的研究和应用  

The Research and Application of Mammary Gland Epithelial Cells

作　　者：闫亚彬[1] 黄英[1] 

机构地区：[1]上海市儿童医院上海交通大学附属儿童医院上海医学遗传研究所,上海200040

出　　处：《实验动物与比较医学》2013年第3期237-242,共6页Laboratory Animal and Comparative Medicine

基　　金：转基因生物新品种培育科技重大专项资助（2009ZX08007-003B）

摘　　要：转基因动物一乳腺生物反应器的研制是近十多年发展起来的一项生物制药方法，具有巨大的应用前景。外源目的基因载体的构建是制备转基因动物一乳腺生物反应器成功的关键技术环节，因此建立简单、快捷检测外源基因载体构建的合理性和表达情况的方法至关重要。由于乳腺上皮细胞能较真实反映哺乳动物乳腺生长发育及泌乳的各项生理功能，开展体外培养哺乳动物乳腺上皮细胞，可作为转基因动物制备过程中载体有效性检验的一种体外模型途径。本文将对哺乳动物乳腺组织的结构和乳腺上皮细胞体外培养的增殖能力、诱导、维持分化的特点，以及乳腺上皮细胞的培养和它在生物、医学领域中的应用研究进行简要概述。Transgenic animal mammary gland bioreactor has been developed for the drugs production as one of the most promising bio-pharmaceutical methods in recent decades. The key technique in the process of the mammary gland bioreactors generation relies on the construction of exogenous gene vectors. It is crucial to establish a simple and quick method to test the accuracy and expression level of constructed gene vectors. As the growth progress and biophysiological lactation functions of mammary glands could be representatively investigated in mammary epithelial cells in vitro, mammary epithelial cells are becoming ideal cell models for detecting the efficacy of constructed-vectors in transgenic animals generations. In this review, it briefly introduced the structure of mammary gland tissue and characteristics of proliferation, differentiation, induction and maintenance of mammary epithelial cells. Additionally, it was summarized the culture approach of mammary epithelial cells and its application in biological and medical researches.

关 键 词：乳腺生物反应器 基因载体 乳腺上皮细胞 体外检测模型 

分 类 号：Q95-33[生物学—动物学]