间歇低氧对自主呼吸大鼠舌下神经电活性长时程易化的影响  

The influence of intermittent hypoxia on long-term facilitation of hypoglossal nerve discharge in spontaneously breathing rats

作　　者：涂学平[1] 王念[1] 胡克[1] 鲜于云艳[1] 曹霞[1] 陈宏斌[1] 康晶[1] 

机构地区：[1]武汉大学人民医院呼吸科,430060

出　　处：《中华结核和呼吸杂志》2013年第6期437-440,共4页Chinese Journal of Tuberculosis and Respiratory Diseases

基　　金：国家自然科学基金资助项目（81070065）

摘　　要：目的探讨间歇低氧对舌下神经电活性长时程易化（LTF）的影响。方法12只成年SD大鼠采用随机数字表法分为慢性间歇低氧组（实验组）及常氧对照组（对照组），每组6只，实验组大鼠放置于间歇低氧动物仓中，对照组放置于常氧动物仓中，每天8h（晨8时至16时），连续4周，随后给予急性问歇低氧刺激，每次5min，共3次，记录两组大鼠急性间歇低氧前后舌下神经放电活性。结果实验组和对照组基础状态下放电频率分别为（73±13）和（58±11）Hz，放电幅度分别为（4．6±1．1）和（3．3±0．7）μV，差异均有统计学意义（均P〈0．05）。经急性间歇低氧干预后．实验组及对照组大鼠舌下神经的放电频率及电活性幅度均明显升高（均P〈0．05）。这种放电水平的增强可持续〉1h，呈典型的长时程易化。实验组在急性低氧处理前、后的放电频率分别为（68±16）和（133±20）Hz，电活性幅度分别为（4．6±1．1）和（8．9±1．4）μV；而对照组在急性间歇低氧处理前、后的放电频率分别为（59±12）和（102±16）Hz，电活性幅度分别为（3．2±0．7）和（4．5±0．7）μV。实验组大鼠经急性间歇低氧干预后呼吸强度的增加幅度明显高于对照组，分别为（408±149）％和（242±31）％，差异有统计学意义（P〈0．05）。结论急性及慢性间歇低氧均可诱导舌下神经长时程易化，而慢性间歇低氧可增强急性问歇低氧诱导的舌下神经长时程易化。Objective To explore the impact of intermittent hypoxia on long-term facilitation （LTF） of hypoglossal nerve discharge. Methods Twelve adult SD rats were divided into the experimental group （ CIH group, n = 6） and the control group （normoxia group, n = 6） by the random number table. The rats in the CIH group were fed in the intermittent hypoxia animal chambers, while the control group was placed in the normoxia animal chambers for 8 h per day （from 8：00 AM to 4：00 PM） for 4 consecutive weeks. After that, 5 minx 3 stimulations of acute intermittent hypoxia （AIH） were administered and the hypoglossal never signals were recorded before and after AIH. Results The baseline frequency and average peak amplitude of hypoglossal nerve discharge in the CIH experimental group were significantly greater than those in the control group. The discharge frequency in the CIH and the control groups was （73 ± 13） Hz, and （58 ± 11 ） Hz, respectively（P 〈0. 05） ; and the discharge amplitude in the 2 groups was （4. 6 ± 1.1 ） μV, and （3.3 ± 0. 7 ） μV, respectively （ P 〈 0. 05 ）. After intervention with AIH, the frequency and the average peak amplitude of the hypoglossal nerve discharge in the experimental and the control groups were significantly increased （all P 〈 0. 05 ）. The increased discharge lasted more than 1 h and this typical phenomenon was referred to as LTF. In the CIH group, the discharge frequency before and after exposure to AIH was （68 ± 16） Hz and （ 133 ±20） Hz,respeetively;and the discharge amplitude was （4. 6 ± 1.1 ） μV and （8.9 ± 1.4）μV, respectively. In the control group, the discharge frequency before and after AIH was （59±12） Hz and （102 ± 16） Hz, respectively; and the discharge amplitude was （3.3 ± 0. 7） μV and （4. 5 ±0.7 ） μV, respectively （P 〈 0.05 ）. After AIH stimulation, the enhanced respiratory intensity of rats in CIH group was much higher than that in the control group [ （408 ± 149）% vs （

关 键 词：间歇低氧 舌下神经 长时程易化 大鼠 

分 类 号：R766[医药卫生—耳鼻咽喉科]