银杏叶提取物对早期中耳放射性损伤防护作用的实验研究  

Experimental study on the therapeutic effect of GBE on earlier mucosa damage in middle ear induced by radiation

作　　者：姜梁[1] 胡晓艳[2] 黎万荣[1] 杨海波[3] 

机构地区：[1]四川泸州医学院附属医院耳鼻咽喉头颈外科,泸州646000 [2]四川泸州医学院病原生物学教研室 [3]四川雅安市人民医院耳鼻咽喉头颈外科

出　　处：《中国中西医结合耳鼻咽喉科杂志》2013年第3期161-164,共4页Chinese Journal of Otorhinolaryngology in Integrative Medicine

摘　　要：目的观察银杏叶提取物对放射性中耳炎动物模型早期中耳放射性损伤的防护作用。方法 44只健康豚鼠,随机分为模型对照1天组、模型对照30天组、银杏叶提取物治疗1天组、银杏叶提取物治疗30天组、空白对照组。以医用直线加速器6MV-X射线照射豚鼠左耳,3.0Gy/次,每周5次,总剂量45Gy。加用银杏叶提取物治疗的豚鼠每次照射前注射EGB0.4ml/kg,模型对照组注射同体积生理盐水,空白对照组不作处理。分别于照射剂量达45Gy后第2天和第30天处死动物,采集标本,行光镜及扫描电镜检查,比较各组豚鼠照射侧中耳黏膜病理改变和超微结构变化。结果射线剂量达45Gy后第2天,见中耳黏膜上皮增厚,炎性细胞浸润,纤毛倒伏、融合,渗出增多;照射后第30天,见中耳黏膜上皮纤毛脱落、减少,损伤加重;在照射前应用EGB的豚鼠,显示中耳黏膜损伤减轻;未用药组较用药组中耳黏膜厚度及白细胞浸润数均增高(均P<0.05)。结论放射线可致中耳黏膜黏液纤毛输送系统功能受损,并随时间推移而加重;银杏提取物可以减轻中耳黏膜的早期放射损伤,并随时间的延长而有进一步的改善。Objective To investigate the therapeutic effect of Ginkgo Biloba extract （GBE） on the earlier mucosa damage in middle ear induced by radiation among animal models. Methods Forty-four adult guinea pigs were randomly divided into 5 groups, i.e. model control group 1 （tested on the 2nd day following radiotherapy ended）, model control group 2 （tested on the 30th day following radiotherapy ended）, EGB treating group 1 （tested on the 2nd day following radiotherapy ended）, EGB treating group 2 （tested on the 30th day following radiotherapy ended） and blank control group. Animals in the first four groups were exposed to X-rays radiation at a total dosage of 45 Gy, which was administered to the left ear of each guinea pig at 3.0 Gy each time and 5 times per week, by use of a linear accelerator 6MV-X. Animals in EGB treating groups were treated with 0.4 ml/kg EGB injected intra- peritoneally 30 rain before radiation each time, while those in model control groups were injected intra-peritoneally with normal saline at the same volume in the same way. Healthy guinea pigs in blank control group were given no any treatment. Tissue samples were taken from the tympanic bullas of animals put into death on the second day and thirtieth day respectively when the radiation period ended for light microscopy and scanning electron microscopy to observe such indexes as mucosa thickness, leukocyte infiltration and the morphology of cilia. Results As seen under light microscope and scanning electron microscope from the modeling animals, middle ear cleft was accumulated with effusion and its mucosa became thicker with inflammatory cells infiltrating and cilia fell off, even fused or collapsed on the 2rid day following the radiation treatment ended. Until the 30th day, such damage in middle ear mucosa became more severe. As a comparison, such damage in middle ear mucosa significantly relieved among the guinea pigs in EGB treating groups, wltF~ statistical significance in the differences of mucosa thickness and infiltrating

关 键 词：中耳黏膜 辐射损伤 银杏叶提取物 疗效 

分 类 号：R285.5[医药卫生—中药学]