microRNA-208在急性心肌梗死病人血清中的变化  

Change of Serum MicroRNA-208 as a Biomarker of Myocardial Injury in the Patients with Acute Myocardial Infarction

作　　者：李祥云[1,2] 蔡成松[1] 潘峰[1] 刘玉华[1] 黄玲玲[1] 楼洪萍[1] 陈兆军[1] 

机构地区：[1]杭州师范大学附属医院检验科,310015 [2]安徽省医学科学研究院

出　　处：《医学研究杂志》2013年第6期62-65,共4页Journal of Medical Research

基　　金：浙江省自然科学基金资助项目(LY12H19001);杭州市医药卫生科技计划项目(20100633B17)

摘　　要：目的探讨血清中microRNA-208(miRNA-208)成为急性心肌梗死(AMI)心肌损伤标志物的可能性和意义。方法收集因急性胸痛发作入院的AMI患者60例和非AMI患者60例,分别在入院即刻和入院后2、4、8、12、24h,2、3、5和7天10个时间点采集血液,利用实时荧光定量PCR法和酶联荧光分析法等方法对血清中miRNA-208、肌红蛋白(Mb)和肌钙蛋白I(cTnI)进行测定,对结果进行统计分析;并对miRNA-208、Mb和cTnI在不同时点的敏感度和特异性进行比较。结果 AMI患者入院即刻血清中miRNA-208表达显著高于对照组(P<0.05),入院后miRNA-208逐渐升高,在入院后12h左右达到高峰;和Mb和cTnI相比,miRNA-208既具有Mb的高度敏感度,又具有cTnI的高度特异性。结论 miRNA-208表达升高可以反映AMI心肌损伤,可能是AMI早期诊断和治疗评估的一种新的检测标志物。Objective To explore the possibility and meaning of serum microRNA - 208 （miRNA - 208） serving as a potential biomarker of myocardial injury for the diagnosis of acute myocardial infarction（AMI）. Methods MiRNA - 208, Mb and cTnI levels were mainly detected with real time fluorescence quantitative PCR （FQ - PCR） and enzyme linked fluorescent analysis methods in 60 AMI and 60 non - AMI patients at ten different time points after chest pain occurring. The results were compared and analyzed with statistical methods. Then the sensitivity and specificity of miRNA - 208, Mb and cTnI were compared at different time points. Results Serum miRNA - 208 level was obviously higher in 60 AMI patients than that in the heahhy controls（ P 〈 0.05 ）. Serum miRNA - 208 level was gradually increased and reached the peak level at about 12h after the admisson of hospital. Compared with Mb and cTnI, miRNA - 208 was as sensitive as Mb and as specific as cTnI. Conclusion The elevated expression of serum miRNA - 208 could reflect myocardial injury of AMI. Maybe it was a new biomarker in the early diagnosis and therapeutic evaluation of AMI.

关 键 词：急性心肌梗死(AMI) microRNA-208(miRNA-208) 肌红蛋白(Mb) 肌钙蛋白I(cTnI) 

分 类 号：R542.22[医药卫生—心血管疾病]