低甲状腺素血症对发育期大鼠行为及海马synaptotagmin 1表达的影响  

Effects of Hypothyroxinemia on Behavior and the Expression of Synaptotagmin1 in Hippocampus of Rats in Development Stage

作　　者：段蔚[1] 陈大庆[2] 钱燕[1] 缪克凡[1] 朱艳可[1] 丁振尧 

机构地区：[1]温州医学院附属第一医院新生儿科,325000 [2]浙江省苍南县人民医院儿科,325800

出　　处：《医学研究杂志》2013年第6期115-119,共5页Journal of Medical Research

基　　金：温州市科技计划项目(Y20110008)

摘　　要：目的研究低甲状腺素血症对发育期大鼠行为及海马内突触结合蛋白1(synaptotagmin 1,syt1)表达量的影响。方法双侧甲状腺切除1日龄(P1)SD新生大鼠建立低甲状腺素血症模型,SD大鼠数字表法分成两组:假手术对照(control,C)组(n=36)和低甲状腺素血症(hypothyroxinemia,HT)组(n=36)。观察大鼠体重变化,P32行高架十字迷宫实验,P34～39行Mor-ris水迷宫实验。检测P10、21、40大鼠甲状腺功能及海马syt1的表达量。结果与C组比较,HT组大鼠体重增长缓慢(P<0.01);HT组大鼠高架十字迷宫进入臂总次数和开臂次数增多(P<0.05),闭臂时间减少(P<0.01);Morris水迷宫实验逃避潜伏期明显延长(P<0.05);HT组P10、21大鼠海马syt1表达量显著增加(P<0.01),P40大鼠海马syt1表达量减少(P<0.05)。结论发育期低甲状腺素血症导致大鼠情感及学习能力改变,并影响其海马syt1的表达量。Objective To observe the effect of hypothyroxinemia on behavior and the expression of synaptotagminl （sytl） in hippocampus during rats development stage. Methods Postnatal day 1 （ P1 ） SD rats were given bilateral thyroidectomy to establish hypothyroxinemia model. Rats were randomly divided into two groups ： control（ C ） group （ n = 36 ） and hypothyroxinemia（HT） group（ n = 36 ）. The weight gain of rats was observed. The elevated plus - maze were employed to detect the anxiety like behaviors at P32 ; and on P34 - 39, rats explored the Morris water maze to be measured spatial learning and memory. The expression of sytl in hippocampus at P10,21,40 was observed. Results Compared with C group, the weight of rats in HT group gained slowly （ P 〈 0.01 ）. In the elevated plus - maze test, the times of entering the total arms and the open - arm markedly in HT group increased （P 〈 0.05） ,and closed arm detention was significantly lower （P 〈 0.01 ）. In the Morris water maze,the latency to find the submerged platform for the HT group was significantly longer than that for the C group（ P 〈 0.05）. The expression of sytl in hippocampus was increased in HT group at P10,21 （ P 〈 0.01 ） , decreased at P40（P 〈 0.05）. Conclusion Hypothyroxinemia in development stage can disturb emotion and learning ability,and change the expression of sytl in hippocampus.

关 键 词：低甲状腺素血症 大鼠 行为 海马内突触结合蛋白1 

分 类 号：R459.7[医药卫生—急诊医学]