拮抗细菌HD-86发酵液稳定性及对相似穿孔线虫的活性  

Stability of the fermentation supernatant from the antagonistic bacterium HD-86 and its activity against Radopholus similis

作　　者：朱莲英[1,2] 符美英[2] 赵志祥[2] 王会芳[2] 陈绵才[2] 

机构地区：[1]海南大学环境与植物保护学院,海口570228 [2]海南省农业科学院农业环境与植物保护研究所,海南省植物病虫害防控重点实验室,海口571100

出　　处：《植物保护》2013年第3期116-119,共4页Plant Protection

基　　金：公益性行业(农业)科研专项(200903040);海南省自然科学基金项目(312081)

摘　　要：利用PCR特异性引物检测了一株对相似穿孔线虫具有高拮抗活性的Burkholderia cepacia菌株HD-86的毒性,结果未检测到该菌株中与人体致病相关的洋葱伯克霍尔德氏菌致病因子(BCESM)毒力基因,从而验证了其对人类和环境的安全性。菌株HD-86发酵上清液对相似穿孔线虫拮抗活性稳定性的研究结果表明,经80℃高温处理60min后其拮抗活性仍然极高,在pH为8～10的范围内随着碱性增强其拮抗性效果越好,紫外线处理对其拮抗活性没有影响。By using PCR specific primers, the potential virulence of Burkholderia cepacia strain HD-86 to human body, which was highly antagonistic against Radopholus similis, was detected. The results indicated that the viru- lence gene of B. cepacia epidemic strain marker （BCESM） did not exist. Thus the strain was deemed safe for hu- man and environment. The results on the stability of fermentation supernatant from the strain HD-86 against R. similes showed that the antagonistic activity remained high at 80℃ for 60 rain, and the antagonistic activity was higher with increasing basicity within the scope of pHS-10. The antagonistic activity was not affected by the ultra- violet ray treatment.

关 键 词：相似穿孔线虫 拮抗细菌 发酵代谢产物 稳定性 

分 类 号：S482.292[农业科学—农药学]