大鼠外周血淋巴细胞2G_y γ射线照射后12h差异基因表达谱的研究  

Study of Differential Gene Expression Profiling in Rat Peripheral Blood Lymphocyte 12 Hours after in-vitro and Whole-body Exposure to 2Gy Gamma Ray

作　　者：尹晶晶[1] 秦秀军[1] 张伟[1] 袁慧[1] 李建国[1] 闻建华[1] 

机构地区：[1]中国辐射防护研究院放射医学与环境医学研究所,山西太原030006

出　　处：《中国辐射卫生》2013年第3期267-269,共3页Chinese Journal of Radiological Health

基　　金：山西省实验动物基金项目(编号:2010k05)

摘　　要：目的通过研究2 Gyγ射线离体和全身照后12 h大鼠外周血淋巴细胞差异基因表达谱的变化情况,在分子水平上为辐射损伤机制的研究提供依据。方法利用基因芯片技术对大鼠离体和全身照射的外周血淋巴细胞进行辐射差异基因筛选和Pathway分析,并利用荧光定量PCR技术对基因芯片结果进行验证。结果离体照射组筛选出差异3倍以上的基因2 001条,全身照射组筛选出差异3倍以上的基因2 590条。两组共同差异3倍以上的基因有312条。离体照射组涉及22个KEGG通路,全身照射组涉及35个KEGG通路,两组有10个共同的KEGG通路。选择其中3条基因进行实时荧光PCR定量分析,得到的相对定量结果与芯片检测结果趋势一致。结论从照后12 h的研究结果中发现,差异表达基因涉及细胞凋亡、细胞周期及信号转导等多个方面,这将为深入研究这些辐射相关基因在淋巴细胞辐射损伤中的作用提供依据。] Objective To evaluate the changes of differential gene expression in peripheral blood lymphocyte of SD rats 12 hours after in - vitro and whole - body exposure to 2Gy ^60Co γ- ray, and provide the evidence for irradiation damage in gene level. Methods The method of microarray was applied to detect the differentially expressed genes and analyze the path- ways. Gene expression was measured using real - time quantitative PCR to validate the results of the mieroarray. Results The results showed that there were 2 001 genes differentially expressed over 3 fold in in - vitro group, 2 590 genes in whole - body group, and 312 genes in both in - vitro and whole - body groups differentially expressed. There were 22 KEGG pathways in in - vitro group, 35 KEGG pathways in whole - body group, and 10 KEGG pathways in both. The relative quantity＇ s results of three genes, selected to validate the microarray, were consistent with microarray results. Conclusion These resuhs indicate that the differentially expressed genes are related to apoptosis, cell cycle and signal transduetion etc, which will provide a basis for the further study of mechanism of irradiation damage.

关 键 词：Γ射线 基因芯片 外周血淋巴细胞 SD大鼠 

分 类 号：Q691.2[生物学—生物物理学]